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US 2019/0194732 A1Published application
Device and method for nucleic acid manipulation
AssigneeGinkgo Bioworks, Inc.
InventorJoseph Jacobson
Life of this patent
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Abstract
Devices and methods are provided for multiplex nucleic acid assembly. Specifically, the device includes a plurality of differentially labeled particles or beads, such that when in use, the labeled particles can barcode different oligonucleotides. The device can be used for nucleic acid singulation during and/or after assembly.
Claims14
A device for multiplex nucleic acid assembly, comprising:
- a) a plurality of differentially labeled particles comprising a first particle, a second particle and a third particle, having a first anchor oligonucleotide, a second anchor oligonucleotide and a third anchor oligonucleotide immobilized thereon, respectively;
- b) a plurality of first construction oligonucleotides designed to at least partially anneal to one another in a first predetermined order, and to at least partially anneal to the first anchor oligonucleotide at a first terminal oligonucleotide, thereby forming a first nucleic acid on the first particle;
- c) a plurality of second construction oligonucleotides designed to at least partially anneal to one another in a second predetermined order, and to at least partially anneal to the second anchor oligonucleotide at a second terminal oligonucleotide, thereby forming a second nucleic acid on the second particle;
- and
- d) a plurality of third construction oligonucleotides designed to at least partially anneal to one another in a third predetermined order, and to at least partially anneal to the third anchor oligonucleotide at a third terminal oligonucleotide, thereby forming a third nucleic acid on the third particle.
2 more elements
A method for multiplex nucleic acid assembly, comprising:
- a) providing a plurality of differentially labeled particles comprising a first particle, a second particle and a third particle, having a first anchor oligonucleotide, a second anchor oligonucleotide and a third anchor oligonucleotide immobilized thereon, respectively;
- b) providing a plurality of first construction oligonucleotides designed to at least partially anneal to one another in a first predetermined order, and to at least partially anneal to the first anchor oligonucleotide at a first terminal oligonucleotide, thereby forming a first nucleic acid on the first particle;
- c) providing a plurality of second construction oligonucleotides designed to at least partially anneal to one another in a second predetermined order, and to at least partially anneal to the second anchor oligonucleotide at a second terminal oligonucleotide, thereby forming a second nucleic acid on the second particle;
- and
- d) providing a plurality of third construction oligonucleotides designed to at least partially anneal to one another in a third predetermined order, and to at least partially anneal to the third anchor oligonucleotide at a third terminal oligonucleotide, thereby forming a third nucleic acid on the third particle.
2 more elements
Dependencies are inferred from the claim text.