Method for detecting active tuberculosis
AssigneeUCL Business PLC
InventorsMahdad NOURSADEGHI, Jennifer ROE, Adrian MARTINEAU
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Abstract
The present invention relates to a method of determining the presence or absence of active tuberculosis in a sample, in particular, comprising determining the levels of one or more biomarkers selected from basic leucine zipper transcription factor ATF-like 2 (BATF2), cluster of differentiation 177 (CD177), haptoglobin (HP), immunoglobulin J chain (IGJ) and galectin 10 (CLC), in said sample. Uses of biomarkers of the invention and kits for performing the method of the invention are also described.
Claims42
A method for determining the presence or absence of active tuberculosis in a sample, the method comprising determining a level of at least one biomarker in the sample, wherein the at least one biomarker is selected from the group consisting of:
- (a) basic leucine zipper transcription factor ATF-like 2 (BATF2)
- (b) cluster of differentiation 177 (CD177);
- (c) haptoglobin (HP);
- (d) immunoglobulin J chain (IGJ);
- and
- (e) galectin 10 (CLC).
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A method of determining whether a subject will be susceptible to a treatment with an anti-tuberculosis agent, said method comprising a step of determining a level of at least one of the following biomarkers in a sample obtained from the subject:
- (a) basic leucine zipper transcription factor ATF-like 2 (BATF2);
- (b) cluster of differentiation 177 (CD177);
- (c) haptoglobin (HP);
- (d) immunoglobulin J chain (IGJ);
- and
- (e) galectin 10 (CLC);
- wherein the level of the one or more biomarkers in the sample as compared to a reference value is indicative of the susceptibility of the subject to treatment with an anti-tuberculosis agent.
A kit for determining the presence or absence of active tuberculosis in a sample from a subject, wherein the kit comprises one or more primer pairs or probes capable of determining a level of at least one biomarker selected from the group consisting of:
- (a) basic leucine zipper transcription factor ATF-like 2 (B ATF2)
- (b) cluster of differentiation 177 (CD177);
- (c) haptoglobin (HP);
- (d) immunoglobulin J chain (IGJ);
- and
- (e) galectin 10 (CLC);
- in said sample;
- and
- wherein the kit optionally comprises a set of instructions.
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A method for determining the presence or absence of active tuberculosis in a sample, said method comprising the steps of:
- (i) determining a level of at least one biomarker in said sample, the at least one biomarker selected from the group consisting of:
- (a) basic leucine zipper transcription factor ATF-like 2 (BATF2);
- (b) cluster of differentiation 177 (CD177);
- (c) haptoglobin (HP);
- (d) immunoglobulin J chain (IGJ);
- and
- (e) galectin 10 (CLC);
- and
- (ii) optionally comparing the level of the at least one biomarker in the sample to a reference value, wherein the level of the at least one biomarker in the sample as compared to the reference value is indicative of the presence or absence of active tuberculosis.
A method for determining whether a subject is suffering from active tuberculosis or a non-tuberculosis infectious disease, the method comprising the steps of:
- (i) determining a level of BATF2 in a sample obtained from the subject;
- (ii) comparing the level of BATF2 in the sample to a reference value;
- wherein a decreased or unchanged level of BATF2 in the sample from the subject compared to the reference value is indicative of the absence of active tuberculosis;
- or
- wherein an increased level of BATF2 in the sample from the subject compared to the reference value necessitates the execution of the following additional method steps:
- (iii) determining the levels of IGJ, FIP, CLC, and CD177 in the sample;
- (iv) comparing the levels of IGJ, HP, CLC, and CD177 in the sample to the corresponding reference values;
- wherein the levels of IGJ, HP, CLC, and CD177 in the sample as compared to the corresponding reference values are indicative of either:
- (a) the presence of active tuberculosis and the absence of a non-tuberculosis infectious disease in the subject;
- or
- (b) the presence of a non-tuberculosis infectious disease and the absence of active tuberculosis in the subject.
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Dependencies are inferred from the claim text.