Composition and methods for processing a sputum sample
AssigneeRoche Molecular Systems, Inc.
InventorsCarolin Bier, Stefan Lange, Pirmin Hans Loetscher
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Abstract
The present disclosure relates to a composition and its use for treating a sputum sample suspected to contain mycobacteria. The composition comprises thymol, a linear or branched alcohol, a chaotropic agent, a reducing agent, a detergent, and a buffer, and has a pH value between 8.5 and 10. Also disclosed is a method for treating a sputum sample suspected to contain mycobacteria.
Claims14
A composition for treating a sputum sample suspected to contain mycobacteria, the composition comprising:
- thymol
- a linear or branched alcohol
- a chaotropic agent
- a reducing agent
- a detergent
- a buffer,
- wherein the composition has a pH value between 8.5 and 10.
A method for treating a sputum sample suspected to contain mycobacteria, the method comprising the following steps:
- a) contacting a sputum sample with a composition comprising thymol
- linear or branched alcohol
- a chaotropic agent
- a reducing agent
- a detergent
- a buffer,
- wherein the composition has a pH value between 8.5 and 10;
- b) mixing the sample with the composition;
- and
- c) incubating the mixture for a period of time and under conditions sufficient for liquefying the sample and inactivating mycobacteria if present in the sample.
Dependencies are inferred from the claim text.