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US 2019/0194706 A1Published application
Biosynthesis of Oxidised 13R-MO and Related Compounds
AssigneeUniversity of Copenhagen
InventorsJohan Andersen-Ranberg, Eirini Pateraki
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AssigneeUniversity of Copenhagen
InventorsJohan Andersen-Ranberg, Eirini Pateraki
Estimating the term…
The invention discloses novel methods for biosynthesis of forskolin and other oxidised 13R-MOs. Oxidised 13R-MO may be valuable 011 its own account or as precursors for production of forskolin. In particular, the invention provides methods of producing an oxidised 13R-manoyl oxide (13R-MO) comprising the steps of providing a host organism comprising a heterologous nucleic acid encoding an enzyme capable of catalysing hydroxylation of 13R-MO and or oxidised 13R-MO at the 9 position, wherein said oxidised 13R-MO carries a —H at the 9-position and incubating said host organism in the presence of I3R-MO under conditions allowing growth of said host organism. The invention also discloses materials for use in said methods, in particular the invention provides the enzyme CYP76AH16.
A method of producing an oxidised 13R-manoyl oxide (13R-MO), the method comprising:
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A host organism comprising a heterologous nucleic acid encoding an enzyme capable of catalysing hydroxylation of 13R-MO and/or oxidised 13R-MO at the 9 position, wherein the oxidised 13R-MO carries a —H at the 9-position.
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A polypeptide having at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein the polypeptide is an enzyme capable of catalysing hydroxylation of 13R-MO and/or oxidised 13R-MO at the 9 position, wherein the oxidised 13R-MO carries a —H at the 9-position at the 9-position.
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Dependencies are inferred from the claim text.