Control of biofilm dispersal for the production of amino acids or amino acid-derived products
AssigneesShanghai Cathay Biotech R&D Center Ltd., Cibt America Inc.
InventorsHoward CHOU, Xiucai LIU
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Abstract
Provided herein is a genetically modified host cell comprising a heterologous nucleic acid encoding a biofilm dispersal polypeptide that decreases intracellular c-di-GMP levels and enhances the production of lysine and lysine derivatives. Further provided are methods of generating such cell and producing lysine and lysine derivatives using the genetically modified host cell.
Claims36
A genetically modified host cell comprising a heterologous nucleic acid encoding a biofilm dispersal polypeptide that decreases intracellular c-di-GMP levels, wherein the host cell overexpresses the biofilm dispersal polypeptide relative to a counterpart host cell that has not been modified to express the heterologous nucleic acid;
- and has at least one additional genetic modification to increase production of an amino acid or an amino acid derivative compared to a wildtype host cell.
A method of engineering a host cell to increase production of an amino acid or an amino acid derivative, the method comprising introduce a heterologous nucleic acid encoding an biofilm dispersal polypeptide that decreases intracellular c-di-GMP levels into the host cell, wherein the host cell has at least one additional genetic modification to increase production of an amino acid or an amino acid derivative compared to a wildtype host cell;
- and
- culturing the host cell under conditions in which the heterologous biofilm dispersal polypeptide is expressed, wherein expression of the biofilm dispersal polypeptide increases the production of an amino acid or an amino acid derivative relative to a counterpart control host cell that has not been modified to express the heterologous nucleic acid.
The method of any one of claims 21 to 27, wherein the biofilm dispersal polypeptide is exogenous to the host cell.
Dependencies are inferred from the claim text.