Acyl-acp thioesterases and mutants thereof
AssigneeCorbion Biotech, Inc.
InventorsJason Casolari, George N. Rudenko, Scott Franklin, Xinhua Zhao
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Abstract
Novel plant acyl-ACP thioesterase genes of the FatB and FatA classes and proteins encoded by these genes are disclosed. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Expression of the novel and/or mutated FATB and FATA genes is demonstrated in oleaginous microalga host cells. Furthermore, a method for producing an oil elevated in one or more of C12:0, C14:0, C16:0, C18:0 and/or C18:1 fatty acids includes transforming a cell with novel and/or mutated FATB and/or FATA genes, e.g., having an N-terminal deletion. The cells produce triglycerides with altered and useful fatty acid profiles.
Claims25
A cDNA or nucleic acid construct comprising a polynucleotide sequence encoding a heterologous regulatory element and a FatB acyl-ACP thioesterase gene operable to produce an altered fatty acid profile in an oil produced by a cell expressing the nucleic acid construct, wherein the FatB gene expresses a protein having an amino acid sequence having at least 65% identity to SEQ ID NO:
- 40 or SEQ ID NO:
- 42.
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A method for producing an oil, the method comprising:
- (a) providing a plastidic, oleaginous cell, optionally a microbial cell, the cell expressing a functional, acyl-ACP thioesterase gene encoded by a FATB gene having a deletion mutation in a region corresponding to the region coding for amino acids 66-98 of SEQ ID NO:
- 8;
- (b) cultivating the cell to produce a cell-oil;
- and
- (c) isolating the cell-oil from the cell.
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-38. (canceled)
Dependencies are inferred from the claim text.