Anaplerotic oil production in microbials
AssigneeHeliae Development, LLC
InventorsENEKO GANUZA TABERNA, MAGDALENA AMEZQUITA ERNULT, CHARLES SELLERS
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Abstract
Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Such compositions can comprise pentadecanoic and heptadecanoic fatty acids. The techniques described herein include methods for producing and deriving such compositions rich in odd-chain fatty acids from microbials, including microalgae and yeasts/fungi.
Claims38
A method for producing anaplerotic oil from microorganisms, the method comprising:
- adding the microorganisms to a culture medium;
- adding propionate to the culture medium resulting in cultured microorganisms;
- and
- producing an anaplerotic oil from the cultured microorganisms, wherein at least five percent of the total fatty acids (TFA) of the anaplerotic oil are odd-chain fatty acids (OCFA), and OCFA make up at least one percent cell dry weight (CDW) of the microbial mass.
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An anaplerotic oil derived from a microbial strain, comprising:
- at least five percent of the anaplerotic oil from the microbial strain's total fatty acid (TFA) content is odd-chain fatty acids (OCFA);
- and
- at least one percent cell dry weight (CDW) of the microbial mass from the microbial strain is odd-chain fatty acids (OCFA);
- wherein the microbial strain is grown in a culture medium comprising added propionate.
22-23. (canceled)
A method for producing anaplerotic oil from microorganisms, the method comprising:
- adding the microorganisms to a culture medium;
- adding a propionate precursor to the culture medium resulting in cultured microorganisms comprising elevated levels of OCFA, at least a portion of the propionate precursor converting to propionate in the culture medium;
- and
- producing an anaplerotic oil from the cultured microorganisms, wherein at least five percent of the total fatty acids (TFA) of the anaplerotic oil are odd-chain fatty acids (OCFA), and OCFA make up at least one percent cell dry weight (CDW) of the microbial mass.
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Dependencies are inferred from the claim text.