Inositol preparation method
AssigneeBonumose, Inc.
InventorsYi-Heng Percival ZHANG, Chun YOU
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Abstract
An inositol preparation method by enzymatic catalysis uses starch and cellulose or substrates thereof as substrates. Raw materials are converted to inositol by in vitro multi-enzyme reaction system in one pot. The yield from the substrate to inositol is significantly improved by process optimization and adding new enzymes. The new enzymes can promote the phosphorolysis of starch or cellulose and utilization of glucose, which is the final production after the phosphorolysis of starch and cellulose. The inositol preparation method described herein has great potentials in industrial production of inositol because of high inositol yield, easy scale-up, low production cost, and lower impact to environment
Claims10
A method for preparing inositol, comprising:
- (1) using starch or starch derivative as substrate, adding α-glucan phosphorylase, phosphoglucomutase, inositol-3-phophate synthase and inositol monophosphatase to establish a multi-enzyme reaction system and perform an enzyme-catalyzed reaction;
- and
- (2) separating and purifying reaction product to obtain inositol.
A method for preparing inositol, comprising:
- (1) using cellulose or cellulose derivative as a substrate, adding cellulase, cellodextrin phosphorylase, cellobiose phosphorylase, phosphoglucomutase, inositol 3-phophate synthase and inositol monophosphatase to establish a multi-enzyme reaction system and perform an enzyme-catalyzed reaction;
- and
- (2) isolating and purifying reaction product to obtain inositol.
Dependencies are inferred from the claim text.