Enzymes and Microorganisms for the Production of 1,3-Butadiene and Other Dienes
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsDavid Nielsen, Shawn Pugh, Rebekah McKenna
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Abstract
A method for the in vivo production of 1,3-butadiene from 2,4-pentadienoate is described (FIG. 1). Enzymes capable of decarboxylating 2,4-pentadienoate to 1,3-butadiene have been discovered. Recombinant expression of these newly discovered enzymes has resulted in the engineering of microorganisms capable of producing 1,3-butadiene when cultured in the presence of 2,4-pentadienoate. 1,3-butadienoate is an important monomer used in the manufacturing of rubbers and plastics. This invention will help to enable the biological production of 1,3-butadiene from renewable resources such as sugar, for example.
Claims25
A method for the production of 1,3-butadiene from a recombinant cell comprising:
- culturing a recombinant host cell with 2,4-pentadienoate, the recombinant host cell expressing at least one gene encoding at least one polypeptide configured for 2,4-pentadienoate activity, wherein expression in the recombinant host cell of the at least one gene encoding at least one polypeptide configured for 2,4-pentadienoate activity provides increased production of 1,3-butadiene in the recombinant host cell relative to a control recombinant host cell lacking expression of the at least one gene encoding the at least one polypeptide configured for 2,4-pentadienoate activity.
A method for the production of 1,3-butadiene from a recombinant cell comprising:
- (i) contacting a recombinant host cell with at least one carbon source, the recombinant host cell including the ability to:
- over produce 2,4-pentadienoate;
- and
- express at least one gene encoding at least one polypeptide having 2,4-pentadienoate decarboxylase activity;
- and
- (ii) culturing said recombinant cell under conditions sufficient to produce 1,3-butadiene, wherein expression of the at least one gene encoding at least one polypeptide having 2,4-pentadienoate decarboxylase activity provides increased production of 1,3-butadiene in the recombinant host cell relative to a control recombinant host cell lacking expression of the at least one gene encoding the at least one polypeptide having 2,4-pentadienoate decarboxylase activity.
An isolated nucleic acid comprising a nucleotide sequence encoding fdc1 from saccharomyces cerevisiae (seq id no:1), wherein expression thereof in a recombinant host cell provides increased production of 1,3-butadiene in the recombinant host cell relative to a control recombinant host cell lacking expression of at least one gene encoding at least one polypeptide having 2,4-pentadienoate decarboxylase activity.
Dependencies are inferred from the claim text.