Vector-Free Delivery of Gene Editing Proteins and Compositions to Cells and Tissues
AssigneeAvectas Limited
InventorsMichael Maguire, Shirley O'Dea
Life of this patent
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Abstract
The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, system, techniques, compositions, and articles are also described.
Claims41
A method for delivering a gene editing composition across a plasma membrane of a cell, comprising:
- providing a population of cells;
- and
- contacting the population of cells with a volume of aqueous solution, the aqueous solution comprising the gene editing composition and an alcohol at a concentration of at least about 2%,
- wherein the volume is a function of:
- (i) exposed surface area of the population of cells;
- or (ii) a number of cells in the population of cells.
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A composition for delivering a payload across a plasma membrane of a cell, the composition comprising an aqueous solution including a payload and an alcohol at a concentration of at least 2%, wherein the payload comprises a gene editing composition.
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A composition for delivering a payload across a plasma membrane of a cell, the composition comprising an aqueous solution including the payload, an alcohol at a concentration of at least 2%, less than about 46 mM of a salt that lacks a magnesium ion, less than about 121 mM sugar, less than 19 mM buffering agent, and about 0.1 to about 10 mM of a magnesium salt, wherein the payload comprises a gene editing composition.
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An apparatus for delivering a gene editing composition across a plasma membrane of a cell, the apparatus comprising:
- a pneumatic generator producing gas under a pressure;
- an atomizer operatively coupled to the pneumatic generator;
- a reservoir configured to contain a volume of aqueous solution, the aqueous solution including the gene editing composition and an alcohol at greater than 2 percent concentration;
- and
- a valve between the pneumatic generator and atomizer, the valve switchable between a closed position for preventing the gas under the pressure from activating the atomizer and an open position for allowing the gas under the pressure to activate the atomizer to produce colloidal droplets from the aqueous solution, the valve having a switching speed less than 250 milliseconds;
- wherein the atomizer is oriented to contact a population of cells with the aqueous solution and wherein the volume is a function of:
- (i) exposed surface area of the population of cells;
- or (ii) a number of cells in the population of cells.
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Dependencies are inferred from the claim text.