Host cells with enhanced protein expression efficiency and uses thereof
AssigneeDevelopment Center for Biotechnology
InventorsHSIN-LIN LU, CHIEN-I LIN, CHAO-YI TENG
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Abstract
A host cell for protein expression having a lower expression level of a gene, as compared to a wild-type cell, wherein the gene is selected from HDAC8, Dab2, Caspase3, Sys1, Ergic3, Grasp, Trim 23, or a combination thereof. The host cells are CHO cells. The lower expression level of the gene results from RNA interference, which may be achieved by transfecting a vector that contains an shRNA targeting the gene.
Claims10
A host cell for protein expression, wherein the host cell comprises a lower expression level of a gene as compared to a wild-type cell, wherein the gene is selected from the group consisting of HDAC8, Dab2, Caspase3, Sys1, Ergic3, Grasp, Trim 23, and a combination thereof.
Dependencies are inferred from the claim text.