US 2019/0194685 A1Published application

Nucleus-Encoded Male Sterility Through Mutation In Cytochrome P450 Oxidase

AssigneeKws Saat AG

InventorsDietrich BORCHARDT, Olaf CZARNECKI, Wolfgang MECHELKE

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Abstract

The invention relates to plants having a nucleus-encoded, recessive, male sterile phenotype and to the gene locus (gst) correlating therewith, including the gene which is responsible for the fertile/sterile phenotype and which is mutated in the sterile phenotype. The invention further provides methods for identifying the genotype correlating with the expression of features indicated above, and the corresponding genetic tools, such as hybridization probes and oligonucleotides. Also described is the use of the plants obtained according to the invention in hybrid breeding and in the production of products obtained from renewable raw materials, such as bioethanol, biogas and sugar-based products.

Claims19

Dependent claims

A plant, in particular a crop plant, that displays a recessive, nucleus-encoded male sterile phenotype, wherein the phenotype correlates to a mutation comprising the endogenic cytochrome P450 oxidase (CYPgst) gene, or to the absence, or low content or activity, of a functional CYPgst protein encoded by the CYPgst wild type gene in comparison with a corresponding (male fertile) wild type plant, characterized in that the CYPgst gene is a gene selected from:

(a) BvCYPgst from Beta vulgaris, subspecies vulgaris, preferably comprising one of the nucleotide sequences in SEQ ID No. 1 or 2, or encodes the amino acid sequence in SEQ ID No. 3, or the homolog, analog or ortholog thereof;
(b) StCYPgst from Solanum tuberosum, preferably comprising one of the nucleotide sequences in SEQ ID No. 12 or 13, or encodes the amino acid sequence in SEQ ID No. 14, or the homolog, analog or ortholog thereof;
(c) ZmCYPgst from Zea mays, preferably comprising one of the nucleotide sequences in SEQ ID No. 9 or 10, or encodes the amino acid sequence in SEQ ID No. 11, or the homolog, analog or ortholog thereof.

A nucleic acid molecule or recombinant DNA molecule comprising a nucleotide sequence selected from the group composed of a:

(a) nucleotide sequence containing the nucleotide sequence in SEQ ID No. 2, or a functional fragment thereof;
(b) nucleotide sequence that encodes the amino acid sequence in SEQ ID No. 3;
(c) nucleotide sequence capable of hybridizing a nucleotide sequence that is complementary to a nucleotide sequence according to (a) or (b) under stringent conditions;
(d) nucleotide sequence that encodes an amino acid sequence, that displays differences to the amino acid sequence in SEQ ID No. 3 in the form of amino acid deletions, substitutions, additions and/or insertions in the amino acid sequence, and is preferably identical to at least 60% of the entire amino acid sequence;
(e) nucleotide sequence that encodes a protein with the same enzymatic activity as the protein encoded by a nucleotide sequence according to one from (a) to (d);
and
(f) nucleotide sequence comprising at least 200 or 400, preferably at least 600 or 800, particularly preferably at least 1,000 consecutive nucleotides from the promoter in the nucleic acid sequence in SEQ ID No. 1, from nucleotide positions 1 to 1518, preferably from nucleotide positions 518 to 1518, particularly preferably from nucleotide positions 1318 to 1518, or a sequence that hybridizes in this region, wherein the nucleotide sequence is able to specifically control the expression of the gene, or a heterologous nucleic acid molecule that is operatively linked to the nucleotide sequence, in flowers and/or fruit.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application: 16/092,613Filed 12 Apr 2017
Priority: DE 10 2016 106 656.712 Apr 2016
Published: 27 Jun 2019
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12N 15/8289
For fertility modification, e.g. apomixis: male sterility
C12N 9/0071
Oxidoreductases: acting on paired donors with incorporation of molecular oxygen
C12N 15/1137
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming…: against enzymes
C12Q 1/6895
For detection or identification of organisms: for plants, fungi or algae