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US 2019/0194683 A1Published application
Methods and compositions for resistance to cyst nematode in plants
AssigneeWisconsin Alumni Research Foundation
InventorsAndrew Farmer Bent, Adam Milton Bayless, Ryan W. Zapotocny
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Abstract
The disclosure relates to methods and compositions for producing plants or plant cells that exhibit improved cyst nematode resistance.
Claims38
A method of producing plant cells with enhanced nematode resistance, comprising:
- a) increasing expression of, altering an expression pattern of, altering a polynucleotide sequence of, altering abundance or localization of a polypeptide product of, or increasing copy number of,
- (i) one or more polynucleotides encoding alpha-soluble N-ethylmaleimide-sensitive factor Attachment Protein (α-SNAP), or resistance-promoting variants thereof, or
- (ii) one or more polynucleotides encoding soluble N-ethylmaleimide-sensitive factor (NSF) proteins, or homologs or variants thereof,
- wherein the plant cells exhibit increased resistance to nematodes.
1 more element
A recombinant expression construct comprising a promoter operably linked to one or more of:
- (i) one or more polynucleotides encoding α-SNAP proteins, or homologs or variants thereof, or
- (ii) one or more polynucleotides encoding NSF proteins, or homologs or variants thereof.
A nematode-resistant transgenic plant cell comprising:
- (i) one or more polynucleotides encoding α-SNAP proteins, or homologs or variants thereof, or
- (ii) one or more polynucleotides encoding NSF proteins, or homologs or variants thereof.
A method of improving growth or survival of a plant cell containing one or more Rhg1 genes conferring nematode resistance, comprising:
- a) increasing expression of, altering an expression pattern of, altering a polynucleotide sequence of, altering abundance or localization of a polypeptide product of, or increasing copy number of,
- (i) one or more polynucleotides encoding α-SNAP proteins, or homologs or variants thereof, or
- (ii) one or more polynucleotides encoding NSF proteins, or homologs or variants thereof.
Dependencies are inferred from the claim text.