Yield improvement in plants
AssigneeKoch Biological Solutions, LLC
InventorsGraham J. Hymus, T. Lynne Reuber, Colleen M. Marion, Oliver J. Ratcliffe, Jeffrey M. Libby
Life of this patent
Estimating the term…
Abstract
Polynucleotides and polypeptides incorporated into expression vectors are introduced into plants and were ectopically expressed. These polypeptides may confer at least one regulatory activity and increased yield, increased light use efficiency, increased photosynthetic capacity, increased photosynthetic rate, increased photosynthetic resource use efficiency, greater vigor, and/or greater biomass as compared to a control plant.
Claims21
A transgenic plant having greater photosynthetic resource use efficiency than a control plant;
- wherein the transgenic plant comprises an exogenous recombinant polynucleotide comprising a photosynthetic tissue-enhanced promoter which is operably linked to a nucleic acid sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 156;
- wherein the photosynthetic tissue-enhanced promoter regulates expression of the polypeptide in a photosynthetic tissue to a level that is effective in conferring greater photosynthetic resource use efficiency in the transgenic plant relative to the control plant;
- wherein the control plant does not comprise the recombinant polynucleotide;
- wherein the photosynthetic tissue-enhanced promoter does not regulate protein expression in a constitutive manner;
- and
- wherein expression of the polypeptide under the regulatory control of the photosynthetic tissue-enhanced promoter confers greater photosynthetic resource use efficiency in the transgenic plant relative to the control plant.
(canceled)
(canceled)
A method for increasing photosynthetic resource use efficiency in a plant, the method comprising:
- (a) providing one or more transgenic plants that comprise an exogenous recombinant polynucleotide comprising a photosynthetic tissue-enhanced promoter operably linked to a nucleic acid sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 156;
- wherein the photosynthetic tissue-enhanced promoter regulates expression of the polypeptide in a non-constitutive manner;
- and
- (b) growing the one or more transgenic plants;
- wherein expression of the polypeptide in the one or more transgenic plants confers increased photosynthetic resource use efficiency relative to a control plant that does not comprise the recombinant polynucleotide.
(canceled)
A method for producing and selecting a crop plant with greater yield or photosynthetic resource use efficiency than a control plant, the method comprising:
- (a) providing one or more transgenic plants that comprise an exogenous recombinant polynucleotide that comprises a photosynthetic tissue-enhanced promoter operably linked to a nucleic acid sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 156, and wherein the photosynthetic tissue-enhanced promoter does not regulate protein expression in a constitutive manner;
- (b) growing a plurality of the transgenic plants;
- and
- (c) selecting a transgenic plant from the step (b) that has greater photosynthetic resource use efficiency than the control plant, wherein the control plant does not comprise the recombinant polynucleotide;
- and wherein expression of the polypeptide in the selected transgenic plant confers the greater yield of the selected transgenic plant relative to the control plant.
Dependencies are inferred from the claim text.