Ap2 domain transcription factor odp2 (ovule development protein 2) and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsWILLIAM J GORDON-KAMM, TIMOTHY G HELENTJARIS, KEITH S LOWE, BO SHEN, MITCHELL C TARCZYNSKI, PEIZHONG ZHENG
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Abstract
Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
Claims11
A method for producing haploid plant embryos comprising providing to a pollen cell, an ODP2 polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence of SEQ ID NO:2, 26, or 28;
- (b) the amino acid sequence having at least 85% sequence identity to SEQ ID NO:2, 26, or 28, wherein said polypeptide has ODP2 activity;
- and,
- thereby inducing embryogenesis.
The method of claim 11, wherein said doubling agent is colchicine.
The method of any of claims 1-12, wherein said haploid plant embryo is a maize haploid plant embryo and said haploid plant cell is a maize haploid plant cell.
Dependencies are inferred from the claim text.