Modified frt recombination site libraries and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsYumin Tao, Dennis Bidney, William J. Gordon-Kamm, Leszek A. Lyznik
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Abstract
Methods and compositions using populations of randomized mod fie FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. Kits comprising the library populations of FRT sites are also provided, as are methods to make a library of modified FRT recombination sites. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest.
Claims9
-15. (canceled)
A plant cell having stably incorporated into its genome at least a first nucleotide sequence of interest flanked by a first and a second recombination site, wherein said first and said second recombination sites are dissimilar and non-recombinogenic with respect to one another;
- such that the genome of the plant cell comprises in the following order:
- the first recombination site, the first nucleotide sequence of interest, and the second recombination site;
- wherein said first and said second recombination sites are selected from the group consisting of:
- FRT1 (SEQ ID NO:39) and FRT6 (SEQ ID NO:41).
Dependencies are inferred from the claim text.