Seedless fruit producing plants
AssigneeNunhems B.V.
InventorsAlberto SIRIZZOTTI, Richard Bernard BERENTSEN, Hendrik Willem VRIEZEN
Life of this patent
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Abstract
The present invention is directed to seedless fruit producing plants. The present invention also comprises methods for production of said plants and the use of nucleic acids encoding cyclin SDS like proteins for the production of seedless fruits.
Claims19
A plant cell having decreased activity of a cyclin SDS like protein compared to a corresponding wild type plant cell, wherein the cyclin SDS like protein of the wild type plant cell is encoded by one of the following nucleic acid molecules:
- a) a nucleic acid molecule, which encodes a protein with the amino acid sequence given under SEQ ID NO:
- 2 or SEQ ID NO:
- 6 or SEQ ID NO:
- 12 or SEQ ID NO:
- 19 or SEQ ID NO:
- 20;
- b) a nucleic acid molecule, which encodes a protein, the sequence of which has an identity of at least 60% with the amino acid sequence given under SEQ ID NO:
- 2 or SEQ ID NO:
- 6 or SEQ ID NO:
- 12 or SEQ ID NO:
- 19 or SEQ ID NO:
- 20;
- c) a nucleic acid molecule, which comprises the nucleotide sequence shown under SEQ ID NO:
- 1 or SEQ ID NO:
- 5 or SEQ ID NO:
- 17 or a complimentary sequence thereof;
- d) a nucleic acid molecule, which has an identity of at least 60% with one of the nucleic acid sequences described under c);
- e) a nucleic acid molecule, which hybridizes with at least one strand of one of the nucleic acid molecules described under a), b), c), or d) under stringent conditions;
- or
- f) a nucleic acid molecule, the nucleotide sequence of which deviates from the sequence of one of the nucleic acid molecules identified under a) or b) due to the degeneration of the genetic code.
A method for production of a plant comprising:
- a) identifying a plant which has a mutation in an allele encoding a cyclin SDS like protein encoding gene wherein the wild type allele of the gene encodes a cyclin SDS like protein comprising at least 60% sequence identity to any one of the following proteins:
- SEQ ID NO:
- 2 or SEQ ID NO:
- 6 or SEQ ID NO:
- 12 or SEQ ID NO:
- 19 or SEQ ID NO:
- 20, and optionally
- b) determining whether the plant is male fertile and whether the plant, or a progeny plant produced by self-fertilization, produces seedless fruits and optionally
- c) selecting a plant comprising at least on copy of the mutant allele of a).
A method for production of a plant comprising:
- a) introducing mutations in a population of plants,
- b) selecting a male fertile plant producing seedless fruit,
- c) verifying if the plant selected under b) has a mutation in an allele encoding a cyclin SDS like protein encoding gene and selecting a plant comprising such a mutation, and optionally
- d) growing/cultivating the plants obtained under c), wherein the wild type allele of the gene encodes a cyclin SDS like protein comprising at least 60% sequence identity to any one of the following proteins:
- SEQ ID NO:
- 2 or SEQ ID NO:
- 6 or SEQ ID NO:
- 12 or SEQ ID NO:
- 19 or SEQ ID NO:
- 20.
A method for production of a plant comprising:
- a) introducing a foreign nucleic acid molecule into a plant, wherein the foreign nucleic acid molecule is
- i) a DNA molecule, which codes at least one antisense RNA, which effects a reduction in the expression of an endogenous gene encoding a cyclin SDS like protein;
- ii) a DNA molecule, which by means of a co-suppression effect leads to the reduction in the expression of an endogenous gene encoding a cyclin SDS like protein;
- iii) a DNA molecule, which codes at least one ribozyme, which splits specific transcripts of an endogenous gene encoding a cyclin SDS like protein;
- iv) a DNA molecule, which simultaneously codes at least one antisense RNA and at least one sense RNA, wherein the said antisense RNA and the said sense RNA form a double-stranded RNA molecule, which effects a reduction in the expression of an endogenous gene encoding a cyclin SDS like protein (RNAi technology);
- v) a nucleic acid molecule introduced by means of in vivo mutagenesis, which leads to a mutation or an insertion of a heterologous sequence in an endogenous gene encoding a cyclin SDS like protein, wherein the mutation or insertion effects a reduction in the expression of a gene encoding a cyclin SDS like protein or results in the synthesis of an inactive cyclin SDS like protein;
- vi) a nucleic acid molecule, which codes an antibody, wherein the antibody results in a reduction in the activity of an endogenous gene encoding a cyclin SDS like protein due to the bonding of the antibody to an endogenous cyclin SDS like protein;
- vii) a DNA molecule, which contains transposons, wherein the integration of these transposons leads to a mutation or an insertion in an endogenous gene encoding a cyclin SDS like protein, which effects a reduction in the expression of an endogenous gene encoding a cyclin SDS like protein, or results in the synthesis of an inactive cyclin SDS like protein;
- viii) a T-DNA molecule, which, due to insertion in an endogenous gene encoding a cyclin SDS like protein, effects a reduction in the expression of an endogenous gene encoding a cyclin SDS like protein, or result in the synthesis of an inactive cyclin SDS like protein;
- or
- ix) a nucleic acid molecule encoding rare-cleaving endonucleases or custom-tailored rare-cleaving endonucleases;
- b) selecting a male fertile plant wherein the plant, or a progeny of the plant produced by self-fertilization, produces seedless fruit, optionally
- c) verifying if the plant selected under b) has a decreased activity of a cyclin SDS like protein compared to wild type plants into whose genome no foreign nucleic acid molecules had been integrated, optionally;
- and
- d) growing/cultivating the plants obtained under c).
(canceled)
Dependencies are inferred from the claim text.