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US 2019/0194670 A1Published application
Bacteria-Based Protein Delivery
AssigneeUniversitäT Basel
InventorsSimon ITTIG, Marlise AMSTUTZ, Christoph KASPER
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Abstract
The present invention relates to recombinant Gram-negative bacterial strains and the use thereof for delivery of repeated domains of a heterologous protein or two or more domains of different heterologous proteins into eukaryotic cells.
Claims23
A recombinant Gram-negative bacterial strain transformed with a vector which comprises in the 5′ to 3′ direction:
- a promoter;
- a first DNA sequence encoding a delivery signal from a bacterial T3SS effector protein, operably linked to said promoter;
- and
- a second DNA sequence encoding repeated domains of a heterologous protein or two or more domains of different heterologous proteins fused in frame to the 3′ end of said first DNA sequence, wherein the heterologous proteins are selected from the group consisting of proteins involved in apoptosis or apoptosis regulation, cell cycle regulators, ankyrin repeat proteins, cell signaling proteins, reporter proteins, transcription factors, proteases, small GTPases, GPCR related proteins, nanobody fusion constructs and nanobodies, bacterial T3SS effectors, bacterial T4SS effectors and viral proteins.
1 more element
A recombinant Gram-negative bacterial strain transformed with a vector which comprises in the 5′ to 3′ direction:
- a first DNA sequence encoding a delivery signal or a fragment thereof from a bacterial effector protein;
- and
- a second DNA sequence encoding repeated domains of a heterologous protein or two or more domains of different heterologous proteins fused in frame to the 3′ end of said first DNA sequence, wherein the heterologous proteins are selected from the group consisting of proteins involved in apoptosis or apoptosis regulation, cell cycle regulators, ankyrin repeat proteins, cell signaling proteins, reporter proteins, transcription factors, proteases, small GTPases, GPCR related proteins, nanobody fusion constructs and nanobodies, bacterial T3SS effectors, bacterial T4SS effectors and viral proteins.
A vector which comprises in the 5′ to 3′ direction:
- a promoter;
- a first DNA sequence encoding a delivery signal from a bacterial T3SS effector protein, operably linked to said promoter;
- a second DNA sequence encoding repeated domains of a heterologous protein or two or more domains of different heterologous proteins fused in frame to the 3′ end of said first DNA sequence, wherein the heterologous proteins are selected from the group consisting of proteins involved in apoptosis or apoptosis regulation, cell cycle regulators, ankyrin repeat proteins, cell signaling proteins, reporter proteins, transcription factors, proteases, small GTPases, GPCR related proteins, nanobody fusion constructs and nanobodies, bacterial T3SS effectors, bacterial T4SS effectors and viral proteins.
A vector which comprises in the 5′ to 3′ direction:
- a first DNA sequence encoding a delivery signal or a fragment thereof from a bacterial effector protein;
- and
- a second DNA sequence encoding repeated domains of a heterologous protein or two or more domains of different heterologous proteins fused in frame to the 3′ end of said first DNA sequence, wherein the heterologous proteins are selected from the group consisting of proteins involved in apoptosis or apoptosis regulation, cell cycle regulators, ankyrin repeat proteins, cell signaling proteins, reporter proteins, transcription factors, proteases, small GTPases, GPCR related proteins, nanobody fusion constructs and nanobodies, bacterial T3SS effectors, bacterial T4SS effectors and viral proteins.
Dependencies are inferred from the claim text.