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AssigneeRegeneron Pharmaceuticals, Inc.
InventorsChris Schoenherr, John McWhirter, Corey Momont, Caitlin Montagna, Lynn Macdonald, Gregg S. Warshaw, Jose F. Rojas, Ka-Man Venus Lai, David M. Valenzuela, Andrew J. Murphy
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Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends.
A method for assembling at least two nucleic acids, comprising:
A method for assembling at least two nucleic acids, comprising:
A method for assembling two or more nucleic acids, comprising:
A method for assembling two or more nucleic acids, comprising:
Dependencies are inferred from the claim text.