Method of producing ginsenosides 20(s)-rg3 and 20(s)-rh2 using ginsenoside glycosidases

A method of producing ginsenoside 20(S)—Rh2 comprising the steps of:

• (a) treating ginseng crude saponin with a ginsenoside glycosidase isolated from Herbiconiux ginsengi, a transformant introduced with a vector comprising a nucleic acid encoding the ginsenoside glycosidase, or a culture of the transformant to prepare a reaction solution comprising ginsenoside 20(S)—Rg3;
• and
• (b) treating the reaction solution prepared in the step (a) with a ginsenoside glycosidase isolated from Flavobacterium johnsoniae, a transformant introduced with a vector comprising a nucleic acid encoding the ginsenoside glycosidase, or a culture of the transformant to produce ginsenoside 20(S)—Rh2.

A composition for converting crude saponin to 20(S)—Rg3, the composition comprising a ginsenoside glycosidase comprising an amino acid sequence represented by SEQ ID NO:

• 3, a transformant introduced with a vector comprising a nucleic acid encoding the ginsenoside glycosidase protein, or a culture of the transformant.