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US 2019/0194653 A1Published application
Crisp-seq, an integrated method for massively parallel single cell rna-seq and crispr pooled screens
AssigneeYeda Research and Development Co. Ltd.
InventorsIdo AMIT, Diego JAITIN, David LARA-ASTIASO, Assaf WEINER, Ido YOFE
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Abstract
An expression construct is disclosed which comprises: (i) a DNA sequence which encodes at least one guide RNA (gRNA) operatively linked to a transcriptional regulatory sequence so as to allow expression of the gRNA in a target cell; (ii) a barcode sequence for identification of the at least one gRNA operatively linked to a transcriptional regulatory sequence so as to allow expression of the barcode sequence in the target cell.
Claims28
An expression construct comprising:
- (i) a DNA sequence which encodes at least one guide RNA (gRNA) operatively linked to a transcriptional regulatory sequence so as to allow expression of said gRNA in a target cell;
- (ii) a barcode sequence for identification of said at least one gRNA operatively linked to a transcriptional regulatory sequence so as to allow expression of said barcode sequence in said target cell.
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Dependencies are inferred from the claim text.