Method and Kit for Viral Vector Isolation
AssigneeGE Healthcare Bio-Sciences AB
InventorTrevor Smith
Life of this patent
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Abstract
The present invention relates to a method for purification of viral vectors, more closely it relates to purification of viral vectors from producer cells by using a single automated process. The method comprises the following steps: a) adding producer cells and cell lysis buffer to a processing container; b) mixing said producer cells and cell lysis buffer in said processing container to obtain a mixture; c) flowing said mixture through a chromatography column for purification of viral vectors, wherein the viral vectors are adsorbed on said chromatography column; and d) eluting viral vectors from the chromatography column into a product container.
Claims13
A method for isolation of viral vectors comprising a gene of interest following production by cultivation of said viral vectors in producer cells, comprising the following steps in a continuous workflow:
- a) adding producer cells and cell lysis buffer to a processing container;
- b) mixing said producer cells and cell lysis buffer in said processing container to form a mixture;
- c) flowing said mixture from the processing container through a chromatography column comprising affinity resin for purification of viral vectors, wherein the viral vectors are adsorbed on said resin in said chromatography column;
- and d) eluting viral vectors from the resin in the chromatography column into a product container.
A kit comprising a processing container, a product container and an affinity column each mounted to a single cassette, and further comprising a source container for producer cells, a cell lysis buffer, a wash buffer, an elution buffer and optionally a waste container located outside the cassette.
Dependencies are inferred from the claim text.