Pectate lyase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsRune Nygaard Monrad, Peter Kamp Hansen, Frank Winther Rasmussen, Anne Vindum Rue, Jens Erik Nielsen, Garry Paul Gippert
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Abstract
The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.
Claims18
A pectate lyase variant, wherein said variant has pectate lyase activity and comprises an alteration at one or more positions selected from the group consisting of:
- 250, 176, 124, 325, 108, 149, 48, 49, 99, 229, 257, 356, 99+176+325, 49+99+176+325+356, 49+99+176+257+325+356, 49+99+176+229+257+325+356, 99+176+325+356, 99+49+176+325+356, 99+124+176+325+356, 48+99+176+325+356, 99+108+176+325+356, 99+49+108+176+325+356, 48+229, 229+257, 229+250, 229+356, 250+257, 257+356, 48+250, 48+356, 250+356, 48+49+99+176+257+325+356, 48+49+99+176+257+325+356, 49+99+124+176+257+325+356, 49+99+124+176+229+250+257+325+356, 49+99+176+229+250+257+325+356, 49+99+108+124+176+229+250+257+325+356, 48+49+99+176+250+257+325+356, 49+99+176+250+325+257+356, 48+257, and 229+250+356, wherein each position corresponds to a position of the amino acid sequence of the pectate lyase having the amino acid sequence of SEQ ID NO:1, and wherein the variant has at least 65%, but less than 100% identity to the amino acid sequence of SEQ ID NO:1.
A pectate lyase variant comprising an alteration at one or more positions selected from the group consisting of positions 250, 176, 124, 108, 149, and 325, wherein numbering is according to SEQ ID NO:
- 1, and wherein the alteration(s) are independently:
- (i) an insertion of an amino acid downstream of the amino acid which occupides the position;
- (ii) a deletion of the amino acid which occupies the position;
- or
- (iii) a substitution of the amino acid which occupies the position with a different amino acid,
- and wherein each position corresponds to a position of the amino acid sequence of the pectate lyase having the amino acid sequence of SEQ ID NO:
- 1, and wherein the variant pectate lyase has at least 75% identity to the amino acid sequence of SEQ ID NO:
- 1.
(canceled)
A method for improving the detergent stability of a parent pectate lyase, comprising:
- introducing into said parent pectate lyase an alteration at one or more positions selected from the group consisting of:
- 48, 49, 99, 108, 124, 149, 176, 229, 250, 257, 325, 356, 99+176+325, 49+99+176+325+356, 49+99+176+257+325+356, 49+99+176+229+257+325+356, 99+176+325+356, 99+49+176+325+356, 99+124+176+325+356, 48+99+176+325+356, 99+108+176+325+356, 99+49+108+176+325+356, 48+229, 229+257, 229+250, 229+356, 250+257, 257+356, 48+250, 48+356, 250+356, 48+49+99+176+257+325+356, 48+49+99+176+257+325+356, 49+99+124+176+257+325+356, 49+99+176+229+257+325+356, 229+250, 229+356, 49+99+124+176+229+250+257+325+356, 49+99+176+229+257+325+356, 49+99+176+229+250+257+325+356, 49+99+124+176+229+250+257+325+356, 49+99+108+124+176+229+250+257+325+356, 48+49+99+176+250+257+325+356, 49+99+176+250+325+257+356, 48+229, 229+257, 229+250, 229+356, 48+257, 250+257, 257+356, 48+250, 48+356, 250+356 and 229+250+356, wherein each position corresponds to a position of the amino acid sequence of the pectate lyase having the amino acid sequence of SEQ ID NO:1, and wherein the altered pectate lyase has at least 75%, but less than 100% sequence identity to the amino acid sequence of SEQ ID NO:1.
A method for improving the detergent stability of a pectate lyase, comprising:
- introducing into said pectate lyase an alteration(s) at one or more positions selected from the group consisting of positions:
- 250, 176, 124, 108, 149, and 325, wherein each position corresponds to a position of the amino acid sequence of the pectate lyase having the amino acid sequence of SEQ ID NO:1, and wherein the pectate lyase (e.g., the altered pectate lyase, e.g., comprising said alteration(s) at one or more positions) has at least 75% sequence identity to the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:2.
Dependencies are inferred from the claim text.