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AssigneesConsejo Superior de Investigaciones Cientificas, Technical University of Darmstadt, Sustainable Momentum, S.L.
InventorsPedro CLAPÉS SABORIT, Karel HERNÁNDEZ SÁNCHEZ, Jesús JOGLAR TAMARGO, Jordi BUJONS VILÁS, Wolf-Dieter FESSNER, Pablo DOMÍNGUEZ DE MARÍA
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The present invention refers to an enzyme consisting of a fusion protein particularly useful as shown through-out the present invention for carrying out the carbon-carbon bond-forming reaction known as the aldol Reaction, preferably for carrying out an aldol reaction by using aldehydes as substrates and preferably pyruvate or a salt thereof, for producing hydroxyketoacids. Said enzyme is made by binding an aldolase to a maltose binding protein. The enzymes display full activity under “highly denaturing” substrate loadings (aldehydes, >1 M).
A composition comprising a fusion protein which in turn comprises a 2-keto-3-deoxy-L-rhamnonate aldolase or a variant thereof, wherein the term “variant” is understood as a protein exhibiting 2-keto-3-deoxy-L-rhamnonate aldolase activity and at least 80% sequence identity with amino acid sequence SEQ ID NO 1 or with an amino acid sequence coded by SEQ ID NO 2 bound to, optionally through a peptide linker, a maltose binding protein (MBP).
Dependencies are inferred from the claim text.