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US 2019/0194635 A1Published application
Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same
AssigneeNovozymes A/S
InventorsSoren Nymand-Grarup, Ninfa Rangel Pedersen, Lorena Palmen Gonzalez, Dan Pettersson, Jens Magnus Eklof, Charlotte Blom, Jesper Salomon
Life of this patent
Estimating the term…
Abstract
The present invention relates to polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. The invention also relates to compositions comprising the polypeptides of the invention and the use of the polypeptides of the invention to solubilise xylan and in animal feed.
Claims21
1-25. (canceled)
A method of solubilising xylan from plant based material, comprising treating plant based material with a GH30 subfamily 8 polypeptide having xylanase activity, wherein the GH30 subfamily 8 polypeptide having xylanase activity is selected from the group consisting of:
- (a) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 60;
- (b) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 6;
- (c) a polypeptide having at least 92% sequence identity to the polypeptide of SEQ ID NO:
- 12;
- (d) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 18;
- (e) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 24;
- (f) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 30;
- (g) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 36;
- (h) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO:
- 42;
- (i) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 48;
- (j) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 54;
- (k) a variant of the polypeptide of SEQ ID NO:
- 60, SEQ ID NO:
- 6, SEQ ID NO:
- 18, SEQ ID NO:
- 24, SEQ ID NO:
- 30, SEQ ID NO:
- 36, SEQ ID NO:
- 42, SEQ ID NO:
- 48 or SEQ ID NO:
- 54 comprising one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49 or 50 positions;
- (l) a variant of the polypeptide of SEQ ID NO:
- 12 comprising one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 or 39 positions;
- (m) a polypeptide comprising the polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k) or (l) and a N-terminal and/or C-terminal His-tag and/or HQ-tag;
- and
- (n) a fragment of a polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), (l) or (m) having at least 90% of the length of the mature polypeptide.
32 more elements
A granule comprising one or more GH30 subfamily 8 polypeptides having xylanase activity, wherein the GH30 subfamily 8 polypeptide having xylanase activity is selected from the group consisting of:
- (a) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 60;
- (b) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 6;
- (c) a polypeptide having at least 92% sequence identity to the polypeptide of SEQ ID NO:
- 12;
- (d) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 18;
- (e) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 24;
- (f) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 30;
- (g) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 36;
- (h) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO:
- 42;
- (i) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 48;
- (j) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 54;
- (k) a variant of the polypeptide of SEQ ID NO:
- 60, SEQ ID NO:
- 6, SEQ ID NO:
- 18, SEQ ID NO:
- 24, SEQ ID NO:
- 30, SEQ ID NO:
- 36, SEQ ID NO:
- 42, SEQ ID NO:
- 48 or SEQ ID NO:
- 54 comprising one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49 or 50 positions;
- (l) a variant of the polypeptide of SEQ ID NO:
- 12 comprising one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 or 39 positions;
- (m) a polypeptide comprising the polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k) or (l) and a N-terminal and/or C-terminal His-tag and/or HQ-tag;
- and
- (n) a fragment of a polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), (l) or (m) having at least 90% of the length of the mature polypeptide.
32 more elements
An isolated polypeptide having xylanase activity, selected from the group consisting of:
- (a) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 6;
- (b) a polypeptide having at least 92% sequence identity to the polypeptide of SEQ ID NO:
- 12;
- (c) a polypeptide having at least 86% sequence identity to the polypeptide of SEQ ID NO:
- 30;
- (d) a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO:
- 36;
- (e) a polypeptide having at least 85% sequence identity to the polypeptide of SEQ ID NO:
- 42;
- (f) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 48;
- (g) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 54;
- (h) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions, or very high stringency conditions with
- (i) the mature polypeptide coding sequence of SEQ ID NO:
- 4,
- (ii) the mature polypeptide coding sequence of SEQ ID NO:
- 10,
- (iii) the mature polypeptide coding sequence of SEQ ID NO:
- 28,
- (iv) the mature polypeptide coding sequence of SEQ ID NO:
- 34,
- (v) the mature polypeptide coding sequence of SEQ ID NO:
- 40,
- (vi) the mature polypeptide coding sequence of SEQ ID NO:
- 46,
- (vii) the mature polypeptide coding sequence of SEQ ID NO:
- 82,
- (viii) the full-length complementary strand of (i), (ii), (iii), (iv), (v), (vi) or (vii);
- (i) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 4;
- (j) a polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 10;
- (k) a polypeptide encoded by a polynucleotide having at least 86% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 28;
- (l) a polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 34;
- (m) a polypeptide encoded by a polynucleotide having at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 40;
- (n) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 46;
- (o) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 52;
- (p) a variant of the polypeptide of SEQ ID NO:
- 6, SEQ ID NO:
- 18, SEQ ID NO:
- 30, SEQ ID NO:
- 42, SEQ ID NO:
- 48 or SEQ ID NO:
- 54 comprising one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49 or 50 positions;
- (q) a variant of the polypeptide of SEQ ID NO:
- 12 or SEQ ID NO:
- 36 comprising one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 or 39 positions;
- (r) a polypeptide comprising the polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), (l), (m), (n), (o), (p) or (q) and a N-terminal and/or C-terminal His-tag and/or HQ-tag;
- and
- (s) a fragment of a polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), (l), (m), (n), (o), (p), (q) or (r) having xylanase activity and having at least 90% of the length of the mature polypeptide.
54 more elements
Dependencies are inferred from the claim text.