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AssigneeAB Enzymes Oy
InventorsKari Juntunen, Leena Valtakari, Susanna Mäkinen, Marika Alapuranen, Hendrik Hellmuth, Pentti Ojapalo, Marc Mennicken, Ulrich Schwaneberg, David Schönauer, Terhi Puranen
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The present invention relates to polypeptides having cellulase activity, in particular to variants derived from the 20K-cellulase enzyme. The invention discloses a number of amino acid residue positions important for the properties of the cellulase enzyme and thereby for the stability and/or performance thereof. The novel variants have improved stability compared to the parental cellulase. In particular, the novel variants have good performance in an antigreying application and excellent stability in the presence of a protease in several detergent compositions even in long-term experiments.
A cellulase variant polypeptide, or an active fragment thereof, wherein the cellulase variant polypeptide or active fragment thereof has cellulase activity and comprises an amino acid sequence having at least 90% identity to residues 22-235 of SEQ ID NO:1 comprising a substitution or deletion at one or more amino acid positions selected from positions 2, 22, 33, 35, 39, 44, 48, 54, 75, 82, 92, 99, 108, 122, 174, 175, 177, 194, 205, 206, 207, 208, 209, 210, 211, 212, 213 or 214, wherein the amino acid positions of said variant polypeptide or active fragment thereof are numbered by correspondence with the mature amino acid sequence of SEQ ID NO:3.
Dependencies are inferred from the claim text.