Compositions, systems and methods for programming immune cell function through targeted gene regulation
AssigneeDuke University
InventorsCharles A. Gersbach, Joseph J. Bellucci
Life of this patent
Estimating the term…
Abstract
Disclosed herein are compositions and methods for programming immune cell function though targeted gene regulation.
Claims42
A DNA targeting system for programming immune cell function, the DNA targeting system comprising a fusion protein and at least one guide RNA (gRNA), the fusion protein comprising two heterologous polypeptide domains, wherein the first polypeptide domain comprises a Clustered Regularly Interspaced Short Palindromic Repeats associated (Cas) protein and the second polypeptide domain comprises a peptide having histone acetyltransferase activity, a peptide having transcription activation activity, or a peptide having transcription repressor activity, wherein the at least one gRNA targets a target region in at least one gene of FoxP3, IL2RA, CTLA4, GATA3, RORC, PDCD1, TNFRSF18, CCR7, CCR4, CXCR3, or TBX21.
A DNA targeting system for programming immune cell function, the DNA targeting system comprising a fusion protein, the fusion protein comprising two heterologous polypeptide domains, wherein the first polypeptide domain comprises a zinc finger protein, a TAL effector, a meganuclease, or a Clustered Regularly Interspaced Short Palindromic Repeats associated (Cas) protein and the second polypeptide domain comprises a peptide having histone acetyltransferase activity, a peptide having transcription activation activity, or a peptide having transcription repressor activity, wherein the at least one gRNA targets a target region in at least one gene of FoxP3, IL2RA, CTLA4, GATA3, RORC, PDCD1, TNFRSF18, CCR7, CCR4, CXCR3, or TBX21.
Dependencies are inferred from the claim text.