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US 2019/0194626 A1Published application
Icp0-mediated enhanced expression system
AssigneeSanofi Pasteur Biologics, LLC
InventorsSimon Delagrave, CharChang Lai
Life of this patent
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Abstract
Methods and compositions for increasing the production of recombinant proteins by introducing ICP0 to cells capable of producing a recombinant protein are encompassed. In one method, the recombinant protein is a protein that is required for the replication of a replication defective virus, wherein the recombinant protein is provided to the replication defective virus in trans.
Claims22
A method for producing a replication defective virus in culture comprising:
- a. providing a cell comprising a nucleic acid encoding at least one recombinant protein, wherein the recombinant protein is required for replication of a replication defective virus;
- b. introducing a herpes simplex virus (HSV) infected cell polypeptide zero (“ICP0”) to the cell by transduction with a virus other than HSV or by transfection, thereby increasing the expression of the recombinant protein;
- c. introducing the replication defective virus to the cell, wherein the recombinant protein complements the replication defective virus in trans;
- d. replicating the replication defective virus in trans;
- and
- e. isolating the replication defective virus.
3 more elements
2-5. (canceled)
(canceled)
A method of increasing recombinant protein expression in a cell in vitro, comprising:
- introducing a herpes simplex virus (HSV) infected cell polypeptide zero (“ICP0”) to the cell by transduction with a virus other than HSV or by transfection, wherein the cell is capable of expressing the recombinant protein;
- and
- isolating the recombinant protein from the cell.
Dependencies are inferred from the claim text.