Use of rna for reprogramming somatic cells
AssigneesBioNTech SE, TRON-Translationale Onkologie an der Universitatsmedizin der Johannes Gutenberg-Univers
InventorsUgur Sahin, Marco Poleganov, Tim Beissert
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Abstract
The present invention provides methods for de-differentiating somatic cells into stem-like cells without generating embryos or fetuses. More specifically, the present invention provides methods for effecting the de-differentiation of somatic cells to cells having stem cell characteristics, in particular pluripotency, by introducing RNA encoding factors inducing the de-differentiation of somatic cells into the somatic cells and culturing the somatic cells allowing the cells to de-differentiate.
Claims12
A RNA-transfected somatic cell population which comprises somatic cells subjected to electroporation, containing in vitro translated mRNA encoding a transcription factor selected from the group consisting of OCT4, SOX2, KLF4, c-MYC, and combinations thereof, and expressing said transcription factor.
A method for producing a RNA-transfected somatic cell population which comprises introducing in vivo translated mRNA into somatic cells by repeated electroporation;
- the introduced mRNA encoding at least one transcription factor which is a member of the group consisting of OCT4, SOX2, KLF4 and c-MYC.
Dependencies are inferred from the claim text.