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AssigneeBaylor College of Medicine
InventorsCliona M. Rooney, Natalia Lapteva Doyle, Sandhya Sharma, Dimitrios Wagner
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Embodiments of the disclosure concern methods and compositions for immunotherapy for diseases and malignancies associated with viruses other than HPV or with non-virus-associated diseases and malignancies, such as wherein the VST encodes a CAR specific for a non-viral cancer and the VST can be stimulated in vitro or in vivo using viruses, viral vaccines or oncolytic viruses. In specific embodiments, methods concern production of immune cells that target one or more antigens of HIV, EBV, CMV, adenovirus, vaccinia virus, and/or VZV, including methods with stimulation steps that employ IL-7 and IL-15, but not IL-2, IL-4, or both. Other specific embodiments utilize stimulations in the presence of certain cells, such as costimulatory cells and certain antigen presenting cells.
A method of producing viral antigen-specific T-cells, comprising the step of stimulating a population of peripheral blood T-cells with antigen presenting cells in the presence of IL-7 and IL-15, wherein the antigen presenting cells are or were previously exposed to a library of peptides, wherein the peptides comprise sequence that corresponds to at least part of the sequence of one or more proteins of one or more viruses that are not HPV, wherein the method comprises one or more of the following:
A method for stimulating T-cells specific for a virus other than HPV, comprising stimulating T-cells specific for the virus with antigen presenting cells in the presence of IL-7 and IL-15 but in the absence of IL-4 and in the presence of co-stimulatory cells, wherein the antigen presenting cells were previously exposed to one or more peptides, wherein the peptides comprise sequence that corresponds to at least part of the sequence of one or more proteins of a virus other than HPV, and wherein the antigen presenting cells are depleted of CD45RA-positive cells.
A method of producing therapeutic T-cells for a virus-associated disease or a non-virus-associated disease, the method comprising the step of stimulating T-cells specific for a virus other than HPV with antigen presenting cells in the presence of one or more of IL-7 and IL-15 but in the absence of IL-4 and in the presence of co-stimulatory cells, wherein the antigen presenting cells were previously exposed to one or more peptides, wherein the peptides comprise sequence that corresponds to at least part of the sequence of one or more proteins of a virus other than HPV, wherein the stimulating produces T-cells therapeutic for the virus-associated disease, wherein the antigen presenting cells are depleted of CD45RA-positive cells.
Dependencies are inferred from the claim text.