Single- and multi-chain chimeric antigen receptors
AssigneeCell Design Labs, Inc.
InventorsPeter Emtage, Sarah Wyman
Life of this patent
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Abstract
Provided herein are single-chain and multi-chain chimeric antigen receptors, nucleic acids encoding the same, and mammalian cells expressing the same. Also provided are methods of treating a cancer in a subject using a mammalian cell expressing any of these single-chain and multi-chain chimeric antigen receptors.
Claims20
A single-chain chimeric antigen receptor, wherein the single-chain chimeric antigen receptor comprises:
- an extracellular antigen-binding domain;
- a transmembrane domain;
- a first intracellular signaling domain from DAP-10 or DAP12;
- a second intracellular signaling domain from a protein selected from the group consisting of:
- 4-1BB, CD27, OX40, CD40, CD28, GITR, CD2, CD5, ICAM-1, CD11a, Lck, TNFR-I, TNFR-II, FasR, CD30, ICOS, LIGHT, NKG2C, and B7-H3;
- and
- an immunoreceptor tyrosine-based activation motif (ITAM).
A multi-chain chimeric antigen receptor, wherein the multi-chain chimeric antigen receptor comprises at least one first polypeptide comprising:
- a transmembrane domain;
- a first intracellular signaling domain from DAP-10 or DAP-12;
- a second intracellular signaling domain from a protein selected from the group consisting of:
- 4-1BB, CD27, OX40, CD40, CD28, GITR, CD2, CD5, ICAM-1, CD11a, Lck, TNFR-I, TNFR-II, FasR, CD30, ICOS, LIGHT, NKG2C, and B7-H3;
- and
- an immunoreceptor tyrosine-based activation motif (ITAM).
A single-chain chimeric antigen receptor, wherein the single-chain chimeric antigen receptor comprises:
- an extracellular antigen-binding domain;
- a transmembrane domain;
- an intracellular signaling domain from a protein selected from the group consisting of:
- 4-1BB, CD27, OX40, CD40, CD28, GITR, DAP-10, DAP-12, CD2, CD5, ICAM-1, CD11a, Lck, TNFR-I, TNFR-II, FasR, CD30, ICOS, LIGHT, NKG2C, and B7-H3;
- and
- an immunoreceptor tyrosine-based activation motif (ITAM).
A multi-chain chimeric antigen receptor, wherein the multi-chain chimeric antigen receptor comprises at least one first polypeptide comprising:
- a transmembrane domain;
- an intracellular signaling domain from a protein selected from the group consisting of:
- 4-1BB, CD27, OX40, CD40, CD28, GITR, DAP-10, DAP-12, CD2, CD5, ICAM-1, CD11a, Lck, TNFR-I, TNFR-II, FasR, CD30, ICOS, LIGHT, NKG2C, and B7-H3;
- and
- an immunoreceptor tyrosine-based activation motif (ITAM).
Dependencies are inferred from the claim text.