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US 2019/0194610 A1Published application
Methods and compositions for in vivo transgenesis
OwnerKevin Esveltinventor
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Abstract
The invention relates, in part, to methods to deliver molecules into in vitro and in vivo cells and into organisms using exogenous and/or endogenous membrane proteins.
Claims32
A composition comprising a host cell, wherein the host cell comprises:
- a) a preselected biomolecule expressed on the surface of the host cell, wherein the expressed preselected exogenous biomolecule is capable of enabling delivery of one or more preselected agents into the host cell;
- and
- b) one or more preselected agents comprising at least one of a:
- nucleic acid molecule, DNA molecule, RNA molecule, nucleic acid-editing molecule, and genome-editing molecule.
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A method of delivering one or more exogenous agents into an oocyte of an original species, the method comprising:
- a) preparing a transgenic oocyte comprising an expressed exogenous low-density lipoprotein (LDL) receptor in a host oocyte of an original species, wherein (i) the exogenous LDL receptor is not naturally expressed in an oocyte of the original species and (ii) the expressed exogenous LDL receptor is displayed on the surface of the transgenic oocyte;
- b) contacting the expressed exogenous LDL receptor with a ligand complex comprising:
- (iii) an LDL receptor ligand molecule that specifically interacts with the expressed exogenous LDL receptor and (iv) one or more preselected agents in association with the LDL receptor ligand molecule, wherein the contacted expressed exogenous LDL receptor is capable of enabling delivery of the one or more preselected agents into the transgenic oocyte;
- and
- c) delivering the one or more preselected agents into the transgenic oocyte displaying the expressed exogenous LDL receptor.
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A method of delivering one or more exogenous agents into an oocyte of an original species, the method comprising:
- a) contacting a host oocyte displaying an expressed endogenous low-density lipoprotein (LDL) receptor on its surface, with a ligand complex comprising:
- (i) an LDL receptor ligand molecule that specifically interacts with the expressed endogenous LDL receptor and (ii) one or more preselected agents in association with the LDL receptor ligand molecule, wherein the contacted expressed endogenous LDL receptor is capable of enabling delivery of the one or more preselected agents into the host oocyte;
- and
- b) delivering the one or more preselected agents into the host oocyte displaying the expressed exogenous LDL receptor on its surface.
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Dependencies are inferred from the claim text.