In vitro 3d culture of human brain tissue
AssigneeThe Jackson Laboratory
InventorMin Tang-Schomer
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Abstract
Provided herein, in some embodiments, are methods comprising culturing human brain tissue spheroids on a three-dimensional scaffold in culture media comprising vascular endothelial growth factor (VEGF), and producing a population of cells, wherein cells of the population are positive for nestin, glial fibrillary acidic protein (GFAP), and/or vimentin.
Claims23
A method comprising culturing human brain tissue spheroids on a three-dimensional scaffold in culture media comprising vascular endothelial growth factor (VEGF), and producing a population of cells, wherein cells of the population are positive for nestin, glial fibrillary acidic protein (GFAP), and/or vimentin.
(canceled)
(canceled)
A composition comprising human brain tissue spheroids, a three-dimensional scaffold, culture media comprising vascular endothelial growth factor (VEGF), and a population of cells, wherein cells of the population are positive for nestin, glial fibrillary acidic protein (GFAP), neuron-specific class III beta-tubulin (TUJI), and/or vimentin.
-34. (canceled)
A method comprising culturing human brain tissue spheroids on a three-dimensional scaffold in culture media comprising vascular endothelial growth factor (VEGF) in the presence of a test agent, and assessing the effect of the test agent on human brain tissue spheroids.
Dependencies are inferred from the claim text.