Compositions and methods comprising serine protease variants

An isolated subtilisin variant of a Bacillus subtilisin, wherein said subtilisin variant is a mature form having proteolytic activity and comprising substitutions selected from:

• S87N/Q109Q/G118D/S128L/P129Q/S130A/S188S/T213E/N248R, S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213R/N248D, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188S/T213T/N248N, S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213E/N248N, S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213E/N248R, S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213T/N248R, S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213T/N248R, S87R/Q109R/G118V/S128L/P129Q/S130A/S188D/T213E/N248R, S87R/Q109Q/G118R/S128L/P129Q/S130A/S188D/T213T/N248R, S87R/Q109R/G118R/S128L/P129Q/S130A/S188D/T213T/N248R, S87N/Q109Q/G118R/S128L/P129Q/S130A/S188D/T213E/N248R, S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213R/N248R, S87R/Q109Q/G118R/S128L/P129Q/S130A/S188S/T213E/N248R, S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213T/N248N, S87R/Q109Q/G118R/S128L/P129Q/S130A/S188D/T213E/N248R, S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213E/N248R, S87D/Q109D/G118D/S128L/P129Q/S130A/S188D/T213E/N248R, S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213R/N248R, S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213T/N248R, S87R/Q109R/G118R/S128L/P129Q/S130A/S188D/T213E/N248R, S87R/Q109Q/G118R/S128L/P129Q/S130A/S188D/T213E/N248N, S87N/Q109R/G118R/S128L/P129Q/S130A/S188D/T213E/N248R, S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213E/N248N, S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213E/N248R, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213T/N248N, S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213R/N248D, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188S/T213T/N248R, S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213R/N248R, S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213T/N248N, S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213T/N248D, S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213R/N248N, S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213E/N248N, S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213T/N248D, S87R/Q109D/G118D/S128L/P129Q/S130A/S188D/T213E/N248R, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213R/N248R, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213R/N248N, S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213R/N248N, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188R/T213E/N248N, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213E/N248N, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188R/T213R/N248R, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213T/N248R, S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213R/N248N, S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213T/N248N, S87R/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213E/N248R, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213E/N248R, S87N/Q109D/G118V/S128L/P129Q/S130A/S188R/T213R/N248N, S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213T/N248D, S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213E/N248D, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188D/T213R/N248D, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188S/T213E/N248N, S87N/Q109R/G118V/S128L/P129Q/S130A/S188D/T213E/N248D, S87R/Q109D/G118R/S128L/P129Q/S130A/S188D/T213E/N248N, S87N/Q109R/G118V/S128L/P129Q/S130A/S188R/T213R/N248R, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188R/T213R/N248N, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188R/T213T/N248R, S87N/Q109Q/G118V/S128L/P129Q/S130A/S188S/T213E/N248R, S87N/Q109R/G118V/S128L/P129Q/S130A/S188S/T213R/N248D, and S87N/Q109Q/G118V/S128L/P129Q/S130A/S188R/T213E/N248R, wherein the positions are numbered by correspondence with the amino acid sequence of B. amyloliquefaciens subtilisin BPN′ set forth as SEQ ID NO:1.

An isolated subtilisin variant of a Bacillus subtilisin, wherein said subtilisin variant is a mature form having proteolytic activity and comprising substitutions selected from:

• A001C/S024V/I107L, A001I/R045T/I107R/A172T, A001K/A172L, A001K/A172W, A001K/I072C/L126F, A001K/I072C/L126F/S164Q, A001K/I072C/V104I/L126F, A001K/I072C/V104I/L126F/S164Q, A001K/I072V, A001K/I072V/L126F, A001K/I072V/L126F/S164F, A001K/I072V/V104I, A001K/I072V/V104I/L126F/S164I, A001K/I072V/V104I/S164F, A001K/I072V/V104I/S164Q, A001K/I107T/A172Q, A001K/I107V/G127Q, A001K/I107V/G127Q/A172Q, A001K/I107V/G127R/A172Q, A001K/L126F, A001K/L126F/S164F, A001K/L126F/S164I, A001K/L126F/S164Q, A001K/R045F/I072C, A001K/R045F/I072C/L126F, A001K/R045F/I072C/V104I/S164F, A001K/R045F/I072V/L126F/S164I, A001K/R045F/I072V/L126F/S164Q, A001K/R045F/I072V/S164F, A001K/R045F/I072V/V104G/S164T, A001K/R045F/I107L/A172N, A001K/R045F/I107T, A001K/R045F/I107V/G127Q, A001K/R045F/L126F/S164F, A001K/R045F/L126F/S164I, A001K/R045F/S164F, A001K/R045F/V104I/L126F/S164Q, A001K/R045K/I072C/L126F/S164Q, A001K/R045K/I072V/L126F, A001K/R045K/I072V/L126F/S164F, A001K/R045K/I072V/L126F/S164Q, A001K/R045K/I072V/S164F, A001K/R045K/I072V/S164Q, A001K/R045K/I072V/V104I/L126F/S164F, A001K/R045K/I072V/V104I/L126F/S164I, A001K/R045K/I072V/V104I/L126F/S164Q, A001K/R045K/I107A/A172T, A001K/R045K/I107R/G127A/A172N, A001K/R045K/I107T/G127R, A001K/R045K/I107T/G127R/A172Q, A001K/R045K/L126F, A001K/R045K/L126F/S164I, A001K/R045K/L126F/S164P, A001K/R045K/L126F/S164Q, A001K/R045K/S164F, A001K/R045K/V104I/S164Q, A001K/R045K/V104L/S164A, A001K/R045N/I107T/G127Q, A001K/R045S/V104C/L126Y/S164F, A001K/S024H/I107T/G127Q, A001K/S024H/I107T/G127R, A001K/S024H/I107V, A001K/S024H/R045N/I107T, A001K/S024H/R045N/I107T/G127Q/A172Q, A001K/S024L/R045A, A001K/S024N/I107V/G127T, A001K/S024N/R045K/A172Y, A001K/S024R/I107T, A001K/S024R/R045N/A172C, A001K/S024R/R045N/I107T, A001K/S024R/R045N/I107T/G127R, A001K/S024T/I107T/G127Q/A172Q, A001K/S024T/R045N/G127Q/A172Q, A001K/S024W/I107T/G127R, A001K/S024W/I107V/G127T/A172Q, A001K/S164Q, A001K/V104E, A001K/V104I/L126F, A001K/V104I/L126F/S164I, A001K/V104I/L126F/S164Q, A001L/I072C, A001L/R045Q/G127H/A172R, A001L/S024D/R045Y/I107Y, A001M/R045S/I107E/A172D, A001P/A172V, A001Q/R045K/I107T, A001R/A172G, A001R/A172Q, A001R/I107T, A001R/I107T/A172Q, A001R/I107T/G127Q/A172Q, A001R/I107V/A172Q, A001R/R045F, A001R/R045K/A172Q, A001R/R045K/G127R, A001R/R045K/I107T/G127Q, A001R/R045K/I107V/G127R, A001R/R045N/A172K, A001R/R045N/A172Q, A001R/R045N/I107T, A001R/R045N/I107T/G127Q, A001R/R045N/I107V/A172V, A001R/R045N/I107V/G127Q/A172Q, A001R/R045N/I107V/G127R, A001R/S024H/I107T, A001R/S024H/R045K/I107T, A001R/S024H/R045K/I107T/A172Q, A001R/S024H/R045N/I107T/A172Q, A001R/S024N, A001R/S024R, A001R/S024R/A172H, A001R/S024R/A172Q, A001R/S024R/I107V/A172L, A001R/S024R/I107V/G127R, A001R/S024R/R045F/I107T/G127Q, A001R/S024R/R045F/I107T/G127R, A001R/S024R/R045K/I107T/G127Q, A001R/S024T/I107T/A172Q, A001R/S024T/R045K/A172Q, A001R/S024T/R045K/I107L/G127P/A172P, A001R/S024T/R045N/I107T/A172Q, A001R/S024W/I107V/A172S, A001R/S024W/R045F/I107V/G127Q, A001R/S024W/R045F/I107V/G127R, A001R/S024W/R045K, A001R/S024W/R045K/I107T/A172Q, A001R/S024W/R045N/I107T, A001S/A172V, A001S/S024L/I107Y/A172S, A001T/S024H/I107L/A172Y, A001V/I107L/A172I, G097P/N185Q/A215I, G097P/Y209W/A215V/S256W, G118L/G127R/S132L, G118T/G127Q, I107A/G127K/A172G, I107C/A172S, I107P/A172S, I107Q/A172D, L126A/S164N, L126F/S164V, M119F/N185R, M119F/N185R/Y209W/S256T, M119F/N185V/Y209W, M119F/S144T/N185V/S256I, M119F/S144W/N185R/S256L, M119F/Y209W, M119F/Y209W/S256I, N018K/A215I, N018K/A215R, N018K/A215V, N018K/G097P/A215I/S256W, N018K/G097P/N185K/S256W, N018K/G097P/N185K/Y209W/A215I/S256W, N018K/G097P/N185Q/Y209W/A215V/S256W, N018K/G097P/N185R/A215R/S256W, N018K/G097P/N185R/A215V/S256W, N018K/G097P/N185R/S256W, N018K/G097P/N185R/Y209W, N018K/G097P/N185R/Y209W/S256W, N018K/G097P/Y209W/A215V/S256W, N018K/N185K, N018K/N185K/A215V/S256W, N018K/N185K/S256W, N018K/N185K/Y209W/A215R, N018K/N185Q/A215R/S256W, N018K/N185Q/A215V/S256W, N018K/N185Q/S256W, N018K/N185Q/Y209W/S256W, N018K/N185R/A215I/S256W, N018K/N185R/A215R/S256W, N018K/N185R/A215V, N018K/N185R/Y209W/A215V/S256W, N018K/S256W, N018Q/G097P/N185K/A215V, N018Q/G097P/N185K/Y209W, N018Q/N185K, N018Q/N185K/S256W, N018Q/N185K/Y209W/A215I/S256W, N018Q/N185K/Y209W/A215V, N018Q/N185K/Y209W/A215V/S256W, N018Q/N185Q/A215V, N018Q/N185Q/S256W, N018Q/N185Q/Y209W/S256W, N018Q/N185R/A215R, N018Q/N185R/S256W, N018R/G097P/N185Q/A215I, N018R/G097P/Y209W/A215V, N018R/G097P/Y209W/S256W, N018R/N185K/A215V/S256W, N018R/N185K/S256W, N018R/N185Q/A215V/S256W, N018R/N185Q/Y209W/S256W, N018R/N185R/A215R, N018R/N185R/S256W, N018R/N185R/Y209W/S256W, N018R/Y209W/A215V/S256W, N018R/Y209W/S256W, N043E/S101E, N043E/S101E/N248K, N043E/S101F, N043E/S101N/N117Y, N043E/S101V, N043E/S101Y/N117I/N248K, N043E/S101Y/N117Y/N248K, N043F/N117I, N043F/S101E/N117I/N248K, N043F/S101R, N043I/N117Y, N043I/S101E/N248I, N043I/S101F/N117Y/N248K, N043I/S101N1N117Y/N248K, N043I/S101R/N248K, N043I/S101Y1N117I/N248I, N043I/S101Y/N117Y/N248K, N043 S/N117Y/N248K, N043 S/N248I, N043 S/S101E, N043 S/S101E/N117I/N248I, N043 S/S101E/N117Y/N248K, N043 S/S101E/N248K, N043 S/S101F, N043 S/S101F/N248K, N043 S/S101N/N117I, N043 S/S101R/N117I/N248K, N043 S/S101V/N117I/N248I, N043 S/S101Y, N043 S/S101Y/N117I, N043 S/S101Y/N117Y, N043 S/S101Y/N248K, N043V/N117Y/N248I, N043V/N248I, N043V/N248K, N043V/S101E/N117I/N248K, N043V/S101E/N248I, N043V/S101F, N043V/S101F/N117I, N043V/S101F/N117Y, N043V/S101F/N248K, N043V/S101R/N117I/N248K, N043V/S101T/N248K, N043V/S101V/N117I, N043V/S101V/N248K, N043V/S101Y, N043V/S101Y/N117I, N043V/S101Y/N117I/N248K, N185K/A215V, N185K/Y209W/S256W, N185Q/A215R/S256W, N185Q/S256W, N185R/S256W, N185R/Y209W/A215I, N185R/Y209W/S256W, N185V/Y209W, P052I/M119F, P052I/M119F/S144T/N185R/Y209W, P052I/M119F/S144V/Y209W, P052I/M119F/S144Y/N185R, P052I/M119F/S144Y/N185R/Y209W/S256I, P052I/M119F/Y209W/S256I, P052I/N185R/Y209W/S256I, P052I/N185V/Y209W, P052I/N185V/Y209W/S256I, P052I/S144T, P052I/S144T/N185R/Y209W/S256T, P052I/S144T/N185V/Y209W/S256T, P052I/S144T/S256I, P052I/S144T/Y209W/S256I, P052I/S144T/Y209W/S256T, P052I/S144V/Y209W, P052I/S144Y/N185R/Y209W, P052I/S144Y/N185V, P052I/S144Y/N185V/S256T, P052I/S144Y/S256T, P052I/S144Y/Y209W/S256I, P052V/M119F/N185V/Y209W/S256I, P052V/M119F/S144T, P052V/M119F/S144T/N185R/S256T, P052V/M119F/S144T/N185R/Y209W, P052V/M119F/S144T/S256T, P052V/M119F/S144T/Y209W, P052V/M119F/S144V/N185V/S256I, P052V/N185V/Y209W/S256I, P052V/S144T, P052V/S144T/N185V/Y209W/S256I, P052V/S144T/N185V/Y209W/S256T, P052V/S144T/Y209W/S256I, P052V/S144V/N185R/Y209W/S256T, P052V/S144V/N185V/Y209W, P052V/S144V/Y209W/S256I, P052V/S144W/N185R, P052V/S144W/N185V/Y209W, P052V/S144W/Y209W, P052V/S144Y/S256T, P052V/S144Y/Y209W, P052V/S256T, Q109A/G118S, Q109D/G118V/G127H, Q109H/G118S/S132P, Q109H/G127R, Q109I/G118R, Q109I/G118S, Q109I/G127C, Q109I/G127T/S132Y, Q109I/S132N, Q109K/G118F/G127Q/S132N, Q109L/G118L/G127Q/S132N, Q109P/G118Q, Q109R/G118V, Q109S/G118R, Q109T/G118N, Q109V/G118F, Q109V/G127Q, Q109V/G127R, Q109V/G127R/S132N, Q109V/G127R/S132Y, R045F/I072V/L126F/S164Q, R045F/I107T, R045K/I072C/V104I/L126F/S164F, R045K/I107T, R045K/L126F/S164F, R045L/I072S/S164I, R045N/I107A/A172R, R045N/I107T/A172Q, R045N/I107V/A172Q, R045S/I107L, R045V/I107R/A172D, S024A/G118I, S024A/Q109Y/G118C, S024C/Q109K/G118I, S024G/G118F/G127R/S132N, S024H/G118K/G127I, S024H/G118L/G127T/S132Y, S024H/G127R, 5024H/I107K, S024H/Q109L, S024H/Q109T/G118R, S024H/Q109V/G127R/S132N, S024H/Q109V/G127T, S024H/S099K/G118F/G127R, S024H/S099K/G118R/G127R/S132H, S024H/S099K/S103P, S024H/S099K/S103P/G118F, S024H/S099K/S103R/G118A/S132P, S024H/S099Q/G118R/G127Q/S132H, S024H/S099Q/G118R/S132H, S024H/S099Q/S103N/G118R/G127T/S132N, S024H/S099Q/S103N/G118T/S132H, S024H/S099Q/S103P, S024H/S099Q/S103P/G118R/S132H, S024H/S099Q/S103P/S132H, S024H/S099Q/S132H, S024H/S099Q/S132N, S024H/S099T/G118R, S024H/S099T/S103N, S024H/S099T/S103N/G127R/S132N, S024H/S099T/S103N/S132N, S024H/S099T/S103P/G118R, S024H/S099T/S103P/G118R/S132H, S024H/S099T/S103P/S132N, S024H/S103N/S132H, S024H/S103P/G127R/S132R, S024H/S132N, S024H/S132R, S0241/1107R/G127I/A172R, S024L/G127R, S024L/Q109K/G127R/S132Y, S024L/Q109T/G118V/G127Q/S132R, S024L/Q109V/G118R/G127Q, S024M/G118V, S024N/G118R/G127L/S132Y, S024N/G127R, S024N/I107T, S024N/Q109T/G127R/S132N, S024N/Q109T/G127T/S132R, S024N/Q109T/G127T/S132Y, S024N/Q109V/G118L/G127Q, S024N/Q109V/G118R/G127T/S132Y, S024N/Q109V/G127R/S132N, S024N/R045K/A172L, S024N/R045K/I107V/A172N, S024N/S099T/G127T/S132H, S024N/S132R, S024P/S099Q/S103P/G118R, S024Q/Q109R/S132R, S024R/G118H/S132V, S024R/G118L/G127R/S132R, S024R/G118R/S132N, S024R/Q109C/G127H, S024R/Q109I/G127T, S024R/Q109K/G118T/G127Q/S132N, S024R/Q109L/G127Q, S024R/Q109R/G118T/G127R, S024R/Q109T/G127R/S132N, S024R/Q109T/G127R/S132Y, S024R/Q109T/G127T/S132Y, S024R/Q109T/S132Y, S024R/Q109V, S024R/Q109V/G118C/S132A, S024R/Q109V/G118R/G127T, S024R/Q109V/G127S, S024R/Q109V/S132N, S024R/R045F/I107T/A172Q, S024R/S132Y, S024T/G118D, S024T/I107T, 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S087N/G118V/S128L/P129Q/S130A/N043T/P052V/S101Y/N248K, S087N/G118V/S128L/P129Q/S130A/N043T/S101E/N248I, S087N/G118V/S128L/P129Q/S130A/N043T/S144V/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/N043V/N185V/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/N043V/P052I, S087N/G118V/S128L/P129Q/S130A/N043V/P052I/S101T/N248K, S087N/G118V/S128L/P129Q/S130A/N043V/P052I/S101Y, S087N/G118V/S128L/P129Q/S130A/N043V/P052V/N248K, S087N/G118V/S128L/P129Q/S130A/N043V/P052V/S101F, S087N/G118V/S128L/P129Q/S130A/N043V/P052V/S101F/N248K, S087N/G118V/S128L/P129Q/S130A/N043V/P052V/S144V/N185R/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/N043V/P052V/S144W/S256T, S087N/G118V/S128L/P129Q/S130A/N043V/P052V/S144Y/N185R/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/N043V/S101E/N248I, S087N/G118V/S128L/P129Q/S130A/N043V/S101E/N248K, S087N/G118V/S128L/P129Q/S130A/N043V/S101F, S087N/G118V/S128L/P129Q/S130A/N043V/S101R, S087N/G118V/S128L/P129Q/S130A/N043V/S101R/N248I, S087N/G118V/S128L/P129Q/S130A/N043V/S101T, S087N/G118V/S128L/P129Q/S130A/N043V/S101T/N248K, S087N/G118V/S128L/P129Q/S130A/N043V/S101Y, S087N/G118V/S128L/P129Q/S130A/N043V/S101Y/N248I, S087N/G118V/S128L/P129Q/S130A/N043V/S144T/N185V/S256I, S087N/G118V/S128L/P129Q/S130A/N043V/S144W/N185V, S087N/G118V/S128L/P129Q/S130A/N043V/S144W/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/N043V/S144Y/N185R/Y209W, S087N/G118V/S128L/P129Q/S130A/N043V/S144Y/N185V/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/N043W/P052V/S101E/N248K, S087N/G118V/S128L/P129Q/S130A/N043W/P052V/S101Y, S087N/G118V/S128L/P129Q/S130A/N043W/P052V/S101Y/N248K, S087N/G118V/S128L/P129Q/S130A/N043W/S101E, S087N/G118V/S128L/P129Q/S130A/N043W/S101F/N248K, S087N/G118V/S128L/P129Q/S130A/N185K/A215V/S256W, S087N/G118V/S128L/P129Q/S130A/N185K/Y209W/A215V, S087N/G118V/S128L/P129Q/S130A/N185K/Y209W/A215V/S256W, S087N/G118V/S128L/P129Q/S130A/N185Q/Y209W/A215R, S087N/G118V/S128L/P129Q/S130A/N185Q/Y209W/A215V/S256W, S087N/G118V/S128L/P129Q/S130A/N185R/A215V/S256W, S087N/G118V/S128L/P129Q/S130A/N185R/S256T, S087N/G118V/S128L/P129Q/S130A/N185R/Y209W, S087N/G118V/S128L/P129Q/S130A/N185R/Y209W/A215V/S256R, S087N/G118V/S128L/P129Q/S130A/N185R/Y209W/S256L, S087N/G118V/S128L/P129Q/S130A/N185V/Y209W, S087N/G118V/S128L/P129Q/S130A/N248I, S087N/G118V/S128L/P129Q/S130A/N248K, S087N/G118V/S128L/P129Q/S130A/P052I/N248K, S087N/G118V/S128L/P129Q/S130A/P052I/S101E/N248K, S087N/G118V/S128L/P129Q/S130A/P052I/S101F/N248K, S087N/G118V/S128L/P129Q/S130A/P052I/S101V/N248K, S087N/G118V/S128L/P129Q/S130A/P052V, S087N/G118V/S128L/P129Q/S130A/P052V/G097T/N185R/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/P052V/S101E/N248I, S087N/G118V/S128L/P129Q/S130A/P052V/S101R, S087N/G118V/S128L/P129Q/S130A/P052V/S144T, S087N/G118V/S128L/P129Q/S130A/P052V/S144T/N185R/S256T, S087N/G118V/S128L/P129Q/S130A/P052V/S144T/N185R/Y209W, S087N/G118V/S128L/P129Q/S130A/P052V/S144T/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/P052V/S144V/N185R/S256I, S087N/G118V/S128L/P129Q/S130A/P052V/S144V/N185R/Y209W, S087N/G118V/S128L/P129Q/S130A/P052V/S144V/N185R/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/P052V/S144V/N185V/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/P052V/S144V/Y209W, S087N/G118V/S128L/P129Q/S130A/P052V/S144Y/N185R/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/P052V/S144Y/N185R/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/P052V/S144Y/N185V, S087N/G118V/S128L/P129Q/S130A/P052V/S144Y/N185V/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/P052V/Y209W, S087N/G118V/S128L/P129Q/S130A/P052V/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/Q109I, S087N/G118V/S128L/P129Q/S130A/Q109L/G127Q, S087N/G118V/S128L/P129Q/S130A/Q109T, S087N/G118V/S128L/P129Q/S130A/Q109T/G127Q, S087N/G118V/S128L/P129Q/S130A/Q109V, S087N/G118V/S128L/P129Q/S130A/R045F/I107T/A172Q, S087N/G118V/S128L/P129Q/S130A/R045K/I072C/L126F, S087N/G118V/S128L/P129Q/S130A/R045N/A172Q, S087N/G118V/S128L/P129Q/S130A/R045N/I107V/A172P, S087N/G118V/S128L/P129Q/S130A/R045S, S087N/G118V/S128L/P129Q/S130A/S024H, S087N/G118V/S128L/P129Q/S130A/S024H/I107V/A172S, S087N/G118V/S128L/P129Q/S130A/S024H/Q109V, S087N/G118V/S128L/P129Q/S130A/S024H/Q109V/G127Q, S087N/G118V/S128L/P129Q/S130A/S024H/R045N/I107T, S087N/G118V/S128L/P129Q/S130A/S024H/S099K/S103N, S087N/G118V/S128L/P129Q/S130A/S024H/S099K/S103N/S132N, S087N/G118V/S128L/P129Q/S130A/S024H/S099Q, S087N/G118V/S128L/P129Q/S130A/S024H/S099Q/S103P, S087N/G118V/S128L/P129Q/S130A/S024H/S099Q/S132N, S087N/G118V/S128L/P129Q/S130A/S024H/S099T/S103P, S087N/G118V/S128L/P129Q/S130A/S024H/S099T/S103P/S132H, S087N/G118V/S128L/P129Q/S130A/S024H/S099T/S103P/S132N, S087N/G118V/S128L/P129Q/S130A/S024H/S099T/S132N, S087N/G118V/S128L/P129Q/S130A/S024H/S103P/S132N, S087N/G118V/S128L/P129Q/S130A/S024L/S099K/S103P/S132N, S087N/G118V/S128L/P129Q/S130A/S024N/I107N, S087N/G118V/S128L/P129Q/S130A/S024N/I107T, S087N/G118V/S128L/P129Q/S130A/S024N/I107V, S087N/G118V/S128L/P129Q/S130A/S024N/Q109K, S087N/G118V/S128L/P129Q/S130A/S024N/Q109T/G127T, S087N/G118V/S128L/P129Q/S130A/S024R, S087N/G118V/S128L/P129Q/S130A/S024R/G127R, S087N/G118V/S128L/P129Q/S130A/S024R/I107T/A172Q, S087N/G118V/S128L/P129Q/S130A/S024R/I107V/A172S, S087N/G118V/S128L/P129Q/S130A/S024R/Q109K, S087N/G118V/S128L/P129Q/S130A/S024R/Q109L, S087N/G118V/S128L/P129Q/S130A/S024R/Q109L/G127Q, S087N/G118V/S128L/P129Q/S130A/S024R/Q109V, S087N/G118V/S128L/P129Q/S130A/S024R/R045F/I107T, S087N/G118V/S128L/P129Q/S130A/S024T, S087N/G118V/S128L/P129Q/S130A/S024T/G127T, S087N/G118V/S128L/P129Q/S130A/S024T/I107T/A172Q, S087N/G118V/S128L/P129Q/S130A/S024T/Q109V/G127T, S087N/G118V/S128L/P129Q/S130A/S024T/R045N/I107V/A172S, S087N/G118V/S128L/P129Q/S130A/S024W, S087N/G118V/S128L/P129Q/S130A/S024W/G127T, S087N/G118V/S128L/P129Q/S130A/S024W/I107T, S087N/G118V/S128L/P129Q/S130A/S024W/Q109I, S087N/G118V/S128L/P129Q/S130A/S024W/Q109L, S087N/G118V/S128L/P129Q/S130A/S024W/Q109R/G127Q, S087N/G118V/S128L/P129Q/S130A/S024W/Q109T, S087N/G118V/S128L/P129Q/S130A/S024W/Q109V, S087N/G118V/S128L/P129Q/S130A/S024W/R045F/I107V/A172Q, S087N/G118V/S128L/P129Q/S130A/S024W/S099K, S087N/G118V/S128L/P129Q/S130A/S024W/S099K/S103P, S087N/G118V/S128L/P129Q/S130A/S024W/S099K/S132H, S087N/G118V/S128L/P129Q/S130A/S024W/S099Q, S087N/G118V/S128L/P129Q/S130A/S024W/S099Q/S103N/G127R/S132N, S087N/G118V/S128L/P129Q/S130A/S024W/S099Q/S103N/S132N, S087N/G118V/S128L/P129Q/S130A/S024W/S099Q/S103P, S087N/G118V/S128L/P129Q/S130A/S024W/S099Q/S132H, S087N/G118V/S128L/P129Q/S130A/S024W/S099Q/S132N, S087N/G118V/S128L/P129Q/S130A/S024W/S099T, S087N/G118V/S128L/P129Q/S130A/S024W/S099T/G127R/S132H, S087N/G118V/S128L/P129Q/S130A/S024W/S099T/S103N, S087N/G118V/S128L/P129Q/S130A/S024W/S099T/S103N/G127R/S132N, S087N/G118V/S128L/P129Q/S130A/S024W/S099T/S103N/S132N, S087N/G118V/S128L/P129Q/S130A/S024W/S099T/S103P/S132H, S087N/G118V/S128L/P129Q/S130A/S024W/S099T/S132N, S087N/G118V/S128L/P129Q/S130A/S099K, S087N/G118V/S128L/P129Q/S130A/S099K/S103N, S087N/G118V/S128L/P129Q/S130A/S099K/S103P, S087N/G118V/S128L/P129Q/S130A/S099K/S132H, S087N/G118V/S128L/P129Q/S130A/S099Q, S087N/G118V/S128L/P129Q/S130A/S099Q/S103N, S087N/G118V/S128L/P129Q/S130A/S099Q/S103P, S087N/G118V/S128L/P129Q/S130A/S099Q/S103P/S132H, S087N/G118V/S128L/P129Q/S130A/S099Q/S132H, S087N/G118V/S128L/P129Q/S130A/S099T/G127Q, S087N/G118V/S128L/P129Q/S130A/S099T/S103N, S087N/G118V/S128L/P129Q/S130A/S099T/S103N/S132H, S087N/G118V/S128L/P129Q/S130A/S099T/S103P, S087N/G118V/S128L/P129Q/S130A/S099T/S103P/S132H, S087N/G118V/S128L/P129Q/S130A/S099T/S132H, S087N/G118V/S128L/P129Q/S130A/S101E, S087N/G118V/S128L/P129Q/S130A/S101E/N248K, S087N/G118V/S128L/P129Q/S130A/S101F/N248K, S087N/G118V/S128L/P129Q/S130A/S101N/N248K, S087N/G118V/S128L/P129Q/S130A/S101R/N248K, S087N/G118V/S128L/P129Q/S130A/S101Y, S087N/G118V/S128L/P129Q/S130A/S103N/S132H, S087N/G118V/S128L/P129Q/S130A/S103P, S087N/G118V/S128L/P129Q/S130A/S144T/N185R, S087N/G118V/S128L/P129Q/S130A/S144T/N185R/S256I, S087N/G118V/S128L/P129Q/S130A/S144T/N185R/Y209W, S087N/G118V/S128L/P129Q/S130A/S144T/N185R/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/S144T/N185V/S256I, S087N/G118V/S128L/P129Q/S130A/S144T/N185V/Y209W, S087N/G118V/S128L/P129Q/S130A/S144T/S256I, S087N/G118V/S128L/P129Q/S130A/S144T/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/S144V, S087N/G118V/S128L/P129Q/S130A/S144V/N185R/S256I, S087N/G118V/S128L/P129Q/S130A/S144V/N185R/Y209W, S087N/G118V/S128L/P129Q/S130A/S144V/N185V, S087N/G118V/S128L/P129Q/S130A/S144V/N185V/S256I, S087N/G118V/S128L/P129Q/S130A/S144V/N185V/Y209W, S087N/G118V/S128L/P129Q/S130A/S144V/Y209W/S256I, S087N/G118V/S128L/P129Q/S130A/S144V/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/S144W/Y209W, S087N/G118V/S128L/P129Q/S130A/S144Y/N185R, S087N/G118V/S128L/P129Q/S130A/S144Y/N185R/Y209W/S256T, S087N/G118V/S128L/P129Q/S130A/S144Y/N185V/Y209W, S087N/G118V/S128L/P129Q/S130A/S164I, S087N/G118V/S128L/P129Q/S130A/S164Q, S087N/G118V/S128L/P129Q/S130A/V104I, S087N/G118V/S128L/P129Q/S130A/V104I/L126F/S164F, S087N/G118V/S128L/P129Q/S130A/V104I/L126F/S164I, S087N/G118V/S128L/P129Q/S130A/V104I/S164I, S087N/G118V/S128L/P129Q/S130A/V104I/S164Q, S087N/G118V/S128L/P129Q/S130A/Y209W, S087N/G118V/S128L/P129Q/S130A/Y209W/A215I/S256W, S087N/G118V/S128L/P129Q/S130A/Y209W/S256W, S099H/S103I/G118N, S099K/G118R, S099K/S103N/G118T, S099K/S103P/G118L/S132N, S099K/S103P/G127Q, S099K/S103P/S132H, S099K/S103R/G118A/S132P, S099N/S132R, S099Q/G118F/G127R, S099Q/G118T/G127Q, S099Q/S103N/G118R/S132N, S099Q/S103N/G118T, S099Q/S103N/G118T/S132H, S099Q/S103N/S132H, S099Q/S103P/G118R, S099Q/S103P/G118R/S132N, S099Q/S103P/S132H, S099Q/S132R, S099T/G118F, S099T/G118R/S132H, S099T/G118T/S132H, S099T/G118T/S132N, S099T/S103P, S099T/S103P/G118L/S132H, S101E/N117I, S101E/N117Y/N248K, S101F/N117I/N248I, S101F/N117Y/N248I, S101F/N117Y/N248K, S101F/N248K, S101R/N117I/N248K, S101R/N117Y, S101R/N248K, S101T/N117Y/N248K, S101T/N248I, S101V/N248K, S101Y/N117I/N248K, S101Y/N117Y, S101Y/N248K, S103G/G118I/G127E/S132E, S103L/S132E, S103R/S132P, S144T/N185R, S144T/N185R/Y209W/S256I, S144T/N185R/Y209W/S256T, S144T/N185V, S144V/N185R/S256I, S144V/N185R/Y209W, S144V/N185R/Y209W/S256T, S144V/S256I, S144V/Y209W, S144W/N185R/Y209W/S256T, S144W/N185V/Y209W/S256I, S144W/S256I, S144Y/Y209W/S256I, V104E/L126I/S164L, wherein the positions are numbered by correspondence with the amino acid sequence of B. amyloliquefaciens subtilisin BPN′ set forth as SEQ ID NO:
• 1.

An isolated subtilisin variant of a Bacillus subtilisin, wherein said subtilisin variant is a mature form having proteolytic activity and comprising substitutions selected from:

• TS87N/G118V/S128L/P129Q/S130A/S24W/S101L/Q109K, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/S101L/Q109R/N185R/A215R, S87N/G118V/S128L/P129Q/S130A/172V/Q109R/S164I, S87N/G118V/S128L/P129Q/S130A/S101Y/Q109R/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/S101Y/Q109L/N185R/A215R, S87R/G118R/S128L/P129Q/S130A/S101K/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/S24W/S101L/Q109L, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/S101Y/Q109R/N185R/A215R, S87N/G118V/S128L/P129Q/S130A/172V/S101L/Q109R/S164I, S87N/G118V/S128L/P129Q/S130A/S101Y/Q109R/S188D/T213E/N248R, S87R/G118R/S128L/P129Q/S130A/172V/S164I/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N76D, S87N/G118V/S128L/P129Q/S130A/S24W/S101Y/Q109K, S87N/G118V/S128L/P129Q/S130A/N43S/P52V/S101R/Q109R, S87N/G118V/S128L/P129Q/S130A/172V/S101Y/Q109R/S164I, S87N/G118V/S128L/P129Q/S130A/S101L/Q109L/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/172V/Q109R/S164I/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N76D/S101L, S87N/G118V/S128L/P129Q/S130A/S24W/S101Y/Q109L, S87N/G118V/S128L/P129Q/S130A/N76D/S164I, S87R/G118R/S128L/P129Q/S130A/N76D/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/S101Y/Q109L/S188D/N248R, S87R/G118R/S128L/P129Q/S130A/V118R/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N76D/S101M, S87N/G118V/S128L/P129Q/S130A/N18K/N76D/G97P/N185R/A215R, S87N/G118V/S128L/P129Q/S130A/172V/S101L/S164I, S87R/G118R/S128L/P129Q/S130A/S101L/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/S101Y/Q109L/S188D/T213E/N248R, S87R/G118R/S128L/P129Q/S130A/Q109L/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/S101L/N185R/A215R, S87N/G118V/S128L/P129Q/S130A/I72V/S101Y/S164I, S87N/G118V/S128L/P129Q/S130A/S101Y/T213E/N248R, S87N/G118V/S128L/P129Q/S130A/I72V/S101L/Q109L/S164I, S87R/G118R/S128L/P129Q/S130A/S101V/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/S101Y/N185R/A215R, S87N/G118V/S128L/P129Q/S130A/I72V/S101V/S164I, S87N/G118V/S128L/P129Q/S130A/S101L/T213E/N248R, S87N/G118V/S128L/P129Q/S130A/I72V/S101Y/Q109L/S164I, S87R/G118R/S128L/P129Q/S130A/S101H/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/Q109R/N185R/A215R, S87N/G118V/S128L/P129Q/S130A/I72V/S101H/S164I, S87N/G118V/S128L/P129Q/S130A/S101L/Q109R/S188D/N248R, S87N/G118V/S128L/P129Q/S130A/N18K/G97P/S101L/Q109L/N185R/A215R, S87R/G118R/S128L/P129Q/S130A/S101M/S188D/N248R, N76D/S87R/G118R/S128L/P129Q/S130A/S188D, N76D/S87R/G118R/S128L/P129Q/S130A/S188D/N248K, S87N/G118V/G127S/S128L/P129Q/S130A, S87N/S101H/G118V/S128L/P129Q/S130A, S87N/S101K/G118V/S128L/P129Q/S130A, S87N/S101V/G118V/S128L/P129Q/S130A, S87N/S101Y/G118V/S128L/P129Q/S130A, S87N/S101L/G118V/S128L/P129Q/S130A, I72V/S87N/G118V/S128L/P129Q/S130A/S164I, wherein the positions are numbered by correspondence with the amino acid sequence of B. amyloliquefaciens subtilisin BPN′ set forth as SEQ ID NO:1.