Gelatin polymer derived from natural sources of cold-adapted marine species and uses thereof

A process to manufacture a composition comprising a solution, which in turn comprises an amino acid chain gelatin polymer comprising a proline content of 20% or less with respect to the total amino acid content and a hydroxyproline content of 20% or less with respect to the total amino acid content, at a concentration from 1% to 20% (w/v), which optionally further comprises a photoinitiator, and wherein the amino acid chain gelatin polymer is chemically functionalized with a chemical agent to become reactive to polymerization or crosslinking in the presence of free radicals, comprising the following steps:

• a. Obtaining an amino acid chain gelatin polymer comprising a proline content of 20% or less with respect to the total amino acid content and a hydroxyproline content of 20% or less with respect to the total amino acid content, and dissolving it in a solvent, preferably PBS or water, to a final concentration between 1% and 20% (w/v), preferably from 5 to 20% (w/v);
• b. Chemically modifying the primary structure of the amino acid chain gelatin polymer of step a), by adding a chemical agent capable of becoming reactive to polymerization or crosslinking in the presence of free radicals;
• c. Removing all unreacted chemical agent from the solution of step b);
• and
• d. Optionally adding a radical-derived initiator or a surfactant, or both.

A process to manufacture a composition comprising a solution which in turn comprises an amino acid chain gelatin polymer comprising a proline content of 20% or less with respect to the total amino acid content and a hydroxyproline content of 20% or less with respect to the total amino acid content wherein the amino acid chain gelatin polymer is from the genus Salmo or Oncorhynchus, at a concentration from 1% to 20% (w/v), which optionally further comprises a photoinitiator, and wherein the amino acid chain is chemically functionalized with a chemical agent selected from the group consisting of methacryloyl groups and acryloyl groups to become reactive to polymerization or crosslinking in the presence of free radicals, comprising the following steps:

• a. obtaining an amino acid chain gelatin polymer comprising a proline content of 20% or less with respect to the total amino acid content and a hydroxyproline content of 20% or less with respect to the total amino acid content wherein the amino acid chain gelatin polymer is from the genus Salmo or Oncorhynchus, and dissolving it in a solvent, to a final concentration between 1% and 20% (w/v);
• b. Chemically modifying the primary structure of the amino acid chain gelatin polymer of step a), by adding methacrylic anhydride to the solution of step a);
• c. Removing all unreacted methacrylic anhydride from the solution of step b);
• d. Optionally adding a radical-derived initiator or a surfactant, or both;
• and
• e. optionally filtering and freeze drying the resultant composition from step c) or d), if applicable.