Single domain binding molecule
AssigneeThe University Court of the University of Aberdeen
InventorsCaroline BARELLE, Mischa Roland MULLER, Valarie CALABRO, Jack BIKKER, John STEVEN, Lioudmila TCHISTIAKOVA, Oleg KOVALENKO, Andrea OLLAND
Life of this patent
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Abstract
The present invention provides a single domain specific binding molecule having the structure FW1-CDR1-FW2-HV2-FW3a-HV4-FW3b-CDR3-FW4 in which the Framework Regions FW1, FW2, FW3a, FW3b, and FW4, the Complementarity Determining Regions CDR1 and CDR3, and the Hypervariable Regions HV2, and HV4 have amino acid sequences as defined which provide a high affinity anti-human serum albumin (HSA) binding domain.
Claims16
A single domain specific binding molecule having the structure
- FW1-CDR1-FW2-HV2-FW3a-HV4-FW3b-CDR3-FW4
- in which the Framework Regions FW1, FW2, FW3a, FW3b, and FW4, the Complementarity Determining Regions CDR1 and CDR3, and the Hypervariable Regions HV2, and HV4 have amino acid sequences in which
- FW1 comprises TRVDQTPRTATRETGESLTINCVLT,
- FW2 comprises TYWYRKNPGS,
- FW3a comprises GRYVESVN,
- FW3b comprises FSLRIKDLTVADSATYICRA,
- FW4 comprises GAGTVLTVN,
- CDR1 comprises DTSYPLYS,
- CDR3 comprises
- (i) MGTNIWTGD,
- (ii) MATNIWTGD, MGTDSWTGD, MGTNSWTGD, MSTNIWTGD, ITTDSWTSD, MGANSWTGD, MGTNGWTGD, SDIAMGTYD, ITTHSWSGD, LSTYMEAGD, MDTSAGVVD,
- (iii) ESPPICTSQGIAAVTKYYD, YTIHIKLEXH, HAGYGVWNRGLQWRGYDXYD, YTPGREDY, EKGRKGSAITSCRRSSYYD, QSLAISTRSYWYD, or
- (iv) GVAGGYCDYALCSSRYAE,
- HV2 comprises SNKEQISIS,
- HV4 comprises KGTKS,
- or a sequence having at least 50% identity thereto, where the amino acid residue “X” represents glutamine (Q).
A single domain specific binding molecule, which has an amino acid sequence selected from the group consisting of:
- E06, BB11 and H08, as shown in FIG. 1, or a sequence having at least 50% identity thereto.
A single domain specific binding molecule, which has an amino acid sequence selected from the group consisting of:
- huE06 v1.1, huH08 v1.1, huE06 v1.2, huE06 v1.3, huE06 v1.4, huE06 v1.5, huE06 v1.6, huE06 v1.7, huE06 v1.8, huE06 v1.9, huE06 v1.10, AC9, AD4, AG11, AH7, BA11, BB10, BC3, BD12, BE4, or BH4, as shown in FIG. 2 or a sequence having at least 50% identity thereto.
Dependencies are inferred from the claim text.