Method for the production of immunoglobulin single variable domains
AssigneeAblynx N.V.
InventorsPeter SCHOTTE, Manu DE GROEVE
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Abstract
Methods are provided for the manufacture of polypeptides comprising at least one immunoglobulin variable domain that result in an increased yield. The methods are based on simultaneous enhancement of one or more auxiliary proteins in the host.
Claims29
A method for the production of a polypeptide comprising or essentially consisting of at least one immunoglobulin single variable domain, said method comprising the step of expressing, in a Pichia host, said polypeptide and simultaneously enhancing, in said Pichia host, the expression of the auxiliary protein HAC1spliced (SEQ ID NO:
- 14).
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A Pichia host that expresses, or that under suitable circumstances is capable of expressing, a polypeptide comprising or essentially consisting of at least one immunoglobulin single variable domain and wherein the expression of the auxiliary protein HAC1spliced (SEQ ID NO:
- 14) is enhanced.
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Dependencies are inferred from the claim text.