Immunotherapy and diagnosis of mucormycosis using coth
AssigneeLos Angeles Biomedical Research Institute at Harbor-UCLA Medical Center
InventorsAshraf S. Ibrahim, Mingfu Liu, Teklegiorgis Ghebremariam, Yue Fu, John E. Edwards, Scott Filler
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Abstract
The invention provided Mucorales CotH antibodies, polypeptides, encoding nucleic acid molecules, and uses thereof. The Mucorales CotH antibodies, polypeptides and encoding nucleic acids disclosed herein can be advantageously used to diagnose, treat or prevent fungal conditions, in particular mucormycosis.
Claims20
An isolated, non-naturally occurring anti-Mucorales CotH antibody, or binding fragment thereof, that (a) specifically binds a peptide having at least 87% sequence identity to amino acids 1 to 15 of SEQ ID NO:
- 39 or a peptide having at least 65% sequence identify to amino acids 1 to 17 of SEQ ID NO:
- 40, and (b) specifically binds to a CotH polypeptide on a fungus of order Mucorales.
An isolated anti-Mucorales CotH antibody, or binding fragment thereof, that (a) specifically binds to a peptide having at least 87% sequence identity to amino acids 1 to 15 of SEQ ID NO:
- 39 or amino acids 1 to 17 of SEQ ID NO:
- 40, (b) specifically binds to a CotH polypeptide on a cell surface of a fungus of genus Rhizopus, Rhizomucor, Lichtheimia, Mucor or Cunninghamella, wherein the CotH polypeptide on said cell surface comprises the peptide, and (c) abrogates endocytosis of the fungus by a mammalian endothelial cell, wherein (i) the antibody is a humanized antibody, a chimeric antibody, a CDR-grafted antibody, or a bifunctional antibody, or (ii) the binding fragment is selected from a single chain antibody, Fab, F(ab′)2, Fd and an Fv fragment.
Dependencies are inferred from the claim text.