US 2019/0194269 A1Published application

Polynucleotides and polypeptides useful for making alkaloid compounds

AssigneesIntrexon Corporation, Serturner Corp.

InventorsPeter James FACCHINI, Joseph E. TUCKER

Life of this patent

Estimating the term…

Abstract

Polynucleotides and polypeptides useful in the manufacture of a class of chemical compounds known as alkaloids are provided. The polynucleotides and polypeptides may be used to synthesize alkaloids, including reticuline, thebaine and morphine, in vivo and in vitro. The polynucleotides further may be used to examine the presence of the polynucleotides in a cell or a cell extract, and to modulate expression thereof in living cells.

Claims49

Dependent claims

A composition comprising an isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of:

(a) SEQ.ID NO:
1, SEQ.ID NO:
2, SEQ.ID NO:
3, SEQ.ID. NO:
4, SEQ.ID NO:
5, SEQ.ID NO:
6, SEQ.ID NO:
7, SEQ.ID. NO:
8, SEQ.ID NO:
9, SEQ.ID NO:
10, SEQ.ID NO:
11, SEQ.ID. NO:
12, SEQ.ID NO:
13, SEQ.ID NO:
14, SEQ.ID NO:
15, SEQ.ID. NO:
16, SEQ.ID NO:
17, SEQ.ID NO:
18, SEQ.ID NO:
19, SEQ.ID. NO:
20, SEQ.ID NO:
21, SEQ.ID NO:
22, and SEQ.ID NO:
23, SEQ.ID NO:
47, SEQ.ID NO:
48, SEQ.ID NO:
49, SEQ.ID. NO:
50, SEQ.ID NO:
51, SEQ.ID NO:
52, SEQ.ID NO:
53, SEQ.ID. NO:
54, SEQ.ID NO:
55, SEQ.ID NO:
56, SEQ.ID NO:
57, SEQ.ID. NO:
58, SEQ.ID NO:
59, SEQ.ID NO:
60, SEQ.ID. NO:
75, SEQ.ID NO:
76, SEQ.ID NO:
77, SEQ.ID NO:
78, SEQ.ID. NO:
79, SEQ.ID NO:
80, SEQ.ID NO:
81, SEQ.ID NO:
82, SEQ.ID NO:
83, SEQ.ID NO:
84, SEQ.ID. NO:
85, SEQ.ID NO:
86, SEQ.ID NO:
87, SEQ.ID NO:
88, SEQ.ID. NO:
89, SEQ.ID NO:
90, SEQ.ID NO:
91, SEQ.ID NO:
92, SEQ.ID NO:
93;
SEQ.ID NO:
113 to SEQ.ID NO:
505 or SEQ.ID. NO:
766;
(b) a nucleic acid sequence that is substantially identical to any one of the nucleic acid sequences of (a);
(c) a nucleic acid sequence that is substantially identical to any one of the nucleic acid sequences of (a) but for the degeneration of the genetic code;
(d) a nucleic acid sequence that is complementary to any one of the nucleic acid sequences of (a);
(e) a nucleic acid sequence encoding a polypeptide having any one of the amino acid sequences set forth in SEQ.ID NO:
24, SEQ.ID NO:
25, SEQ.ID NO:
26, SEQ.ID. NO:
27, SEQ.ID NO:
28, SEQ.ID NO:
29, SEQ.ID NO:
30, SEQ.ID. NO:
31, SEQ.ID NO:
32, SEQ.ID NO:
33, SEQ.ID NO:
34, SEQ.ID. NO:
35, SEQ.ID NO:
36, SEQ.ID NO:
37, SEQ.ID NO:
38, SEQ.ID. NO:
39, SEQ.ID NO:
40, SEQ.ID NO:
41, SEQ.ID NO:
42, SEQ.ID. NO:
43, SEQ.ID NO:
44, SEQ.ID NO:
45, SEQ.ID NO:
46, SEQ.ID NO:
61, SEQ.ID NO:
62, SEQ.ID NO:
63, SEQ.ID NO:
64, SEQ.ID. NO:
65, SEQ.ID NO:
66, SEQ.ID NO:
67, SEQ.ID NO:
68, SEQ.ID. NO:
69, SEQ.ID NO:
70, SEQ.ID NO:
71, SEQ.ID NO:
72, SEQ.ID. NO:
73, SEQ.ID NO:
74, SEQ.ID. NO:
94, SEQ.ID NO:
95, SEQ.ID NO:
96, SEQ.ID NO:
97, SEQ.ID. NO:
98, SEQ.ID NO:
99, SEQ.ID NO:
100, SEQ.ID NO:
101, SEQ.ID NO:
102, SEQ.ID NO:
103, SEQ.ID. NO:
104, SEQ.ID NO:
105, SEQ.ID NO:
106, SEQ.ID NO:
107, SEQ.ID. NO:
108, SEQ.ID NO:
109, SEQ.ID NO:
110, SEQ.ID NO:
111, SEQ.ID NO:
112, SEQ.ID NO:
506 to SEQ.ID NO:
765 or SEQ.ID NO:
768 to SEQ.ID NO:
901;
(f) a nucleic acid sequence that encodes a functional variant of any one of the amino acid sequences set forth in SEQ.ID NO:
24, SEQ.ID NO:
25, SEQ.ID NO:
26, SEQ.ID. NO:
27, SEQ.ID NO:
28, SEQ.ID NO:
29, SEQ.ID NO:
30, SEQ.ID. NO:
31, SEQ.ID NO:
32, SEQ.ID NO:
33, SEQ.ID NO:
34, SEQ.ID. NO:
35, SEQ.ID NO:
36, SEQ.ID NO:
37, SEQ.ID NO:
38, SEQ.ID. NO:
39, SEQ.ID NO:
40, SEQ.ID NO:
41, SEQ.ID NO:
42, SEQ.ID. NO:
43, SEQ.ID NO:
44, SEQ.ID NO:
45, SEQ.ID NO:
46, SEQ.ID NO:
61, SEQ.ID NO:
62, SEQ.ID NO:
63, SEQ.ID NO:
64, SEQ.ID. NO:
65, SEQ.ID NO:
66, SEQ.ID NO:
67, SEQ.ID NO:
68, SEQ.ID. NO:
69, SEQ.ID NO:
70, SEQ.ID NO:
71, SEQ.ID NO:
72, SEQ.ID. NO:
73, SEQ.ID NO:
74, SEQ.ID. NO:
94, SEQ.ID NO:
95, SEQ.ID NO:
96, SEQ.ID NO:
97, SEQ.ID. NO:
98, SEQ.ID NO:
99, SEQ.ID NO:
100, SEQ.ID NO:
101, SEQ.ID NO:
102, SEQ.ID NO:
103, SEQ.ID. NO:
104, SEQ.ID NO:
105, SEQ.ID NO:
106, SEQ.ID NO:
107, SEQ.ID. NO:
108, SEQ.ID NO:
109, SEQ.ID NO:
110, SEQ.ID NO:
111, SEQ.ID NO:
112, SEQ.ID NO:
506 to SEQ.ID NO:
765 or SEQ.ID NO:
768 to SEQ.ID NO:
901;
and
(g) a nucleic acid sequence that hybridizes under stringent conditions to any one of the nucleic acid sequences set forth in (a), (b), (c), (d), (e) or (f).

185 more elements

Dependencies are inferred from the claim text.

Facts

Application: 16/312,895Filed 27 Jun 2017
Priority: 27 Jun 2016
Published: 27 Jun 2019
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants
C12N 9/00
Enzymes
C12N 15/52
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: genes encoding for enzymes or proenzymes
C12N 15/80
Vectors or expression systems specially adapted for eukaryotic hosts: for fungi

Family

Application62/355,022Us provisional application · filed 27 Jun 2016
Application62/433,431Us provisional application · filed 13 Dec 2016
Application62/514,104Us provisional application · filed 2 Jun 2017
US 11,479,586 B2Related patent
US 2019/0194269 A1This patent

From the US filing history. Foreign family members aren’t listed.

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Citations

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Abstract

A composition comprising an isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of:

(a) SEQ.ID NO:
1, SEQ.ID NO:
2, SEQ.ID NO:
3, SEQ.ID. NO:
4, SEQ.ID NO:
5, SEQ.ID NO:
6, SEQ.ID NO:
7, SEQ.ID. NO:
8, SEQ.ID NO:
9, SEQ.ID NO:
10, SEQ.ID NO:
11, SEQ.ID. NO:
12, SEQ.ID NO:
13, SEQ.ID NO:
14, SEQ.ID NO:
15, SEQ.ID. NO:
16, SEQ.ID NO:
17, SEQ.ID NO:
18, SEQ.ID NO:
19, SEQ.ID. NO:
20, SEQ.ID NO:
21, SEQ.ID NO:
22, and SEQ.ID NO:
23, SEQ.ID NO:
47, SEQ.ID NO:
48, SEQ.ID NO:
49, SEQ.ID. NO:
50, SEQ.ID NO:
51, SEQ.ID NO:
52, SEQ.ID NO:
53, SEQ.ID. NO:
54, SEQ.ID NO:
55, SEQ.ID NO:
56, SEQ.ID NO:
57, SEQ.ID. NO:
58, SEQ.ID NO:
59, SEQ.ID NO:
60, SEQ.ID. NO:
75, SEQ.ID NO:
76, SEQ.ID NO:
77, SEQ.ID NO:
78, SEQ.ID. NO:
79, SEQ.ID NO:
80, SEQ.ID NO:
81, SEQ.ID NO:
82, SEQ.ID NO:
83, SEQ.ID NO:
84, SEQ.ID. NO:
85, SEQ.ID NO:
86, SEQ.ID NO:
87, SEQ.ID NO:
88, SEQ.ID. NO:
89, SEQ.ID NO:
90, SEQ.ID NO:
91, SEQ.ID NO:
92, SEQ.ID NO:
93;
SEQ.ID NO:
113 to SEQ.ID NO:
505 or SEQ.ID. NO:
766;
(b) a nucleic acid sequence that is substantially identical to any one of the nucleic acid sequences of (a);
(c) a nucleic acid sequence that is substantially identical to any one of the nucleic acid sequences of (a) but for the degeneration of the genetic code;
(d) a nucleic acid sequence that is complementary to any one of the nucleic acid sequences of (a);
(e) a nucleic acid sequence encoding a polypeptide having any one of the amino acid sequences set forth in SEQ.ID NO:
24, SEQ.ID NO:
25, SEQ.ID NO:
26, SEQ.ID. NO:
27, SEQ.ID NO:
28, SEQ.ID NO:
29, SEQ.ID NO:
30, SEQ.ID. NO:
31, SEQ.ID NO:
32, SEQ.ID NO:
33, SEQ.ID NO:
34, SEQ.ID. NO:
35, SEQ.ID NO:
36, SEQ.ID NO:
37, SEQ.ID NO:
38, SEQ.ID. NO:
39, SEQ.ID NO:
40, SEQ.ID NO:
41, SEQ.ID NO:
42, SEQ.ID. NO:
43, SEQ.ID NO:
44, SEQ.ID NO:
45, SEQ.ID NO:
46, SEQ.ID NO:
61, SEQ.ID NO:
62, SEQ.ID NO:
63, SEQ.ID NO:
64, SEQ.ID. NO:
65, SEQ.ID NO:
66, SEQ.ID NO:
67, SEQ.ID NO:
68, SEQ.ID. NO:
69, SEQ.ID NO:
70, SEQ.ID NO:
71, SEQ.ID NO:
72, SEQ.ID. NO:
73, SEQ.ID NO:
74, SEQ.ID. NO:
94, SEQ.ID NO:
95, SEQ.ID NO:
96, SEQ.ID NO:
97, SEQ.ID. NO:
98, SEQ.ID NO:
99, SEQ.ID NO:
100, SEQ.ID NO:
101, SEQ.ID NO:
102, SEQ.ID NO:
103, SEQ.ID. NO:
104, SEQ.ID NO:
105, SEQ.ID NO:
106, SEQ.ID NO:
107, SEQ.ID. NO:
108, SEQ.ID NO:
109, SEQ.ID NO:
110, SEQ.ID NO:
111, SEQ.ID NO:
112, SEQ.ID NO:
506 to SEQ.ID NO:
765 or SEQ.ID NO:
768 to SEQ.ID NO:
901;
(f) a nucleic acid sequence that encodes a functional variant of any one of the amino acid sequences set forth in SEQ.ID NO:
24, SEQ.ID NO:
25, SEQ.ID NO:
26, SEQ.ID. NO:
27, SEQ.ID NO:
28, SEQ.ID NO:
29, SEQ.ID NO:
30, SEQ.ID. NO:
31, SEQ.ID NO:
32, SEQ.ID NO:
33, SEQ.ID NO:
34, SEQ.ID. NO:
35, SEQ.ID NO:
36, SEQ.ID NO:
37, SEQ.ID NO:
38, SEQ.ID. NO:
39, SEQ.ID NO:
40, SEQ.ID NO:
41, SEQ.ID NO:
42, SEQ.ID. NO:
43, SEQ.ID NO:
44, SEQ.ID NO:
45, SEQ.ID NO:
46, SEQ.ID NO:
61, SEQ.ID NO:
62, SEQ.ID NO:
63, SEQ.ID NO:
64, SEQ.ID. NO:
65, SEQ.ID NO:
66, SEQ.ID NO:
67, SEQ.ID NO:
68, SEQ.ID. NO:
69, SEQ.ID NO:
70, SEQ.ID NO:
71, SEQ.ID NO:
72, SEQ.ID. NO:
73, SEQ.ID NO:
74, SEQ.ID. NO:
94, SEQ.ID NO:
95, SEQ.ID NO:
96, SEQ.ID NO:
97, SEQ.ID. NO:
98, SEQ.ID NO:
99, SEQ.ID NO:
100, SEQ.ID NO:
101, SEQ.ID NO:
102, SEQ.ID NO:
103, SEQ.ID. NO:
104, SEQ.ID NO:
105, SEQ.ID NO:
106, SEQ.ID NO:
107, SEQ.ID. NO:
108, SEQ.ID NO:
109, SEQ.ID NO:
110, SEQ.ID NO:
111, SEQ.ID NO:
112, SEQ.ID NO:
506 to SEQ.ID NO:
765 or SEQ.ID NO:
768 to SEQ.ID NO:
901;
and
(g) a nucleic acid sequence that hybridizes under stringent conditions to any one of the nucleic acid sequences set forth in (a), (b), (c), (d), (e) or (f).

185 more elements

Facts

Application

16/312,895Filed 27 Jun 2017

Priority

27 Jun 2016

Published

27 Jun 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants
C12N 9/00
Enzymes
C12N 15/52
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: genes encoding for enzymes or proenzymes
C12N 15/80
Vectors or expression systems specially adapted for eukaryotic hosts: for fungi

Family

Application62/355,022Us provisional application · filed 27 Jun 2016

Application62/433,431Us provisional application · filed 13 Dec 2016

Application62/514,104Us provisional application · filed 2 Jun 2017

US 11,479,586 B2Related patent

US 2019/0194269 A1This patent

From the US filing history. Foreign family members aren’t listed.