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US 2019/0194247 A1Published application
Bone protein extraction without demineralization
AssigneeRensselaer Polytechnic Institute
InventorsTimothy Paul Cleland, Deepak Vashishth
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Abstract
Cell culture devices, and related methods and kits, for modeling isotropic-to-anisotropic cellular transitions are provided. The devices can include a substrate having an isotropic film surface with one or more regions of aligned fibers dispersed thereon.
Claims6
An extraction buffer comprising:
- ammonium phosphate dibasic;
- or ammonium phosphate dibasic and ammonium bicarbonate;
- or ammonium phosphate dibasic, ammonium bicarbonate, and guanidine HCl;
- or sodium phosphate dibasic and sodium bicarbonate;
- or sodium phosphate dibasic, sodium bicarbonate, and guanidine HCl;
- or potassium phosphate dibasic and potassium bicarbonate;
- or potassium phosphate dibasic, potassium bicarbonate, and guanidine HCl.
4 more elements
Dependencies are inferred from the claim text.