Convective pcr device

A device for quantitative polymerase chain reaction by thermal convection through a reagent container, wherein the reagent container is loaded with a reactant and a reagent, in which the reagent comprises a fluorescent dye or a probe, wherein the device comprises:

• (1) a first frame disposed on a horizontal plane and has a first perforation hole, a fan, and a venting hole, wherein the first frame has a first upper surface and a first lower surface, and a heating coil and a first temperature sensor are disposed on the lower surface;
• (2) a second frame disposed under the first frame, substantially parallel to the horizontal plane, the second frame having a second perforation hole, the second frame having an second upper surface and a second lower surface, wherein the second frame further comprises a clamping groove disposed on the second lower surface, and the clamping groove is substantially parallel to the horizontal plane and is connected to the second perforation hole;
• (3) a glass device, disposed in the clamping groove, the glass device comprising an glass upper surface, a glass lower surface and a contact node, wherein a transparent conductive film is disposed on the glass upper surface or the glass lower surface, and a size of the glass device is substantially the same as a size of the clamping groove and the glass device is fixed to the clamping groove by using the glass upper surface or the glass lower surface, and the contact node is disposed at the same side of the glass device coated with the transparent conductive film;
• (4) a power supply device for supplying power to the contact node to heat the glass device;
• (5) a light source for exciting the fluorescent dye or the probe;
• (6) a photon receiver for detecting and receiving fluorescent signals;
• (7) a processor for processing and regulating system;
• and
• (8) an accommodating space disposed between the first frame and the second frame for accommodating the reagent container, wherein the reagent container passes across the first perforation hole and penetrates into the accommodating space, and further passes through the second perforation hole and the clamping groove for contacting the glass upper surface,
• wherein the power supply device supplies a current to the contact node to start heating the glass device, and when the glass device is heated to a predetermined temperature, the polymerase chain reaction starts, and the fluorescent dye or the probe is excited by the light source, and the fluorescent dye or the probe emits a light with specific wavelength, which is measured by the photon receiver, and the result is feedback to the processor,
• wherein if environmental temperature measured by the first temperature sensor is higher than a predetermined temperature range, the measured environmental temperature will be feedback to the processor, the processor then turns on the fan to lower the temperature, until the measured temperature is cooled to a predetermined environmental range, the result of the first temperature sensor will be feedback to the processor, which then stops the fan;
• if environmental temperature measured by the first temperature sensor is lower than a predetermined temperature range, the measured temperature will be feed backed to the processor, the processor then turns on the heating coil to increase temperature, until the measured temperature is heated to the predetermined environmental range, the result of the first temperature sensor will be feedback to the processor, which then stops the heating coil.