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US 2019/0185907 A1Published application
Continuous glucose monitoring using an fad-dependent glucose dehydrogenase
AssigneeKikkoman Corporation
InventorYosuke Masakari
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Abstract
Provided is a method of continuous glucose monitoring (CGM) comprising using an FAD-GDH. The FAD-GDH is capable of retaining initial activity over a certain period of time. Also provided is a method for screening for an FAD-GDH suitable for use in CGM as well as a CGM device comprising an FAD-GDH.
Claims13
A method of continuous glucose monitoring comprising using a GDH capable of
- (a) retaining about 60% or more of the initial activity over a period of 1 week when retained at about 37-40° C. in solution,
- (b) retaining about 40% or more of the initial activity over a period of 2 weeks when retained at about 37-40° C. in solution, or
- (c) retaining about 30% or more of the initial activity over a period of 3 weeks when retained at about 37-40° C. in solution,
- wherein said GDH is an FAD dependent GDH and wherein said GDH is not a membrane bound protein.
1 more element
A method for screening a GDH to be used in continuous glucose monitoring, said method comprising the steps of:
- (i) preparing a candidate GDH,
- (ii) retaining said candidate GDH at about 30-40° C. in solution for a predetermined period,
- (iii) determining the residual activity of said GDH after step (ii),
- (iv) comparing the residual activity determined in step (iii) with the initial activity of said candidate GDH,
- wherein, when the residual GDH activity is
- (a) about 60% or more compared to the initial GDH activity when retained at about 30-40° C. in solution over a period of 1 week,
- (b) about 40% or more compared to the initial GDH activity when retained at about 30-40° C. in solution over a period of 2 weeks, or
- (c) about 30% or more compared to the initial GDH activity when retained at about 30-40° C. in solution over a period of 3 weeks,
- then said candidate GDH is selected as a GDH having the potential to be used in continuous glucose monitoring over a period of 1 to 3 weeks.
6 more elements
A continuous glucose monitoring device comprising a GDH capable of
- (a) retaining about 60% of the initial activity over a period of 1 week when retained at about 37-40° C. in solution,
- (b) retaining about 40% of the initial activity over a period of 2 weeks when retained at about 37-40° C. in solution, or
- (c) retaining about 30% of the initial activity over a period of 3 weeks when retained at about 37-40° C. in solution,
- wherein said GDH is an FAD dependent GDH and wherein said GDH is not a membrane bound protein.
1 more element
Dependencies are inferred from the claim text.