Production and use of phosphoethanolamine cellulose and derivatives
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsLynette Cegelski, Wiriya Thongsomboon
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Abstract
Phosphoethanolamine cellulose and methods of making and using it are disclosed. In particular, the invention relates to a method of producing a phosphoethanolamine cellulose biosynthetically using a BcsG phosphoethanolamine transferase for cellulose modification. Recombinant constructs encoding BcsG are described, including constructs encoding BcsG by itself or in combination with BcsE and BcsF, which increase the extent of cellulose modification and the amount of modified cellulose produced. Production of phosphoethanolamine cellulose in cell culture and derivatization of phosphoethanolamine cellulose are also described.
Claims38
A cellulose-producing host cell comprising a recombinant polynucleotide encoding a BcsG phosphoethanolamine transferase operably linked to a promoter.
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A composition comprising a phosphoethanolamine cellulose ester, wherein at least one hydroxyl group of the phosphoethanolamine cellulose is esterified.
A composition comprising a phosphoethanolamine cellulose ether, wherein at least one hydroxyl group of the phosphoethanolamine cellulose is etherified.
A composition comprising a phosphoethanolamine cellulose, wherein at least one amine group is modified.
A method of hydrolyzing a phosphoethanolamine cellulose, the method comprising contacting the phosphoethanolamine cellulose with one or more cellulases.
Dependencies are inferred from the claim text.