Nucleosidase
AssigneesAmano Enzyme Inc., Suntory Holdings Limited
InventorsKeita Okuda, Toru Katase, Yuko Kuritani, Naoki Matsumoto, Tomoko Fujimura, Ikuma Mizuguchi, Takako Inui
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Abstract
It is an object of the present invention to provide a novel enzyme useful for producing low-purine foods or beverages. There is disclosed a nucleosidase comprising an amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 85% or more identity with the amino acid sequence, or an amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having 88% or more identity with the amino acid sequence.
Claims18
A nucleosidase comprising an amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence having 85% or more identity with the amino acid sequence, or an amino acid sequence of SEQ ID NO:
- 2 or an amino acid sequence having 88% or more identity with the amino acid sequence.
A nucleosidase having the following enzymological properties:
- (1) action:
- catalyzing a reaction of hydrolyzing purine nucleosides into D-ribose and purine bases and showing activity even in the presence of adenosine, adenine, inosine, hypoxanthine, guanosine, guanine, and xanthine;
- (2) molecular weight:
- about 49 kDa (by SDS-PAGE) when the nucleosidase does not contain N-linked oligosaccharides;
- (3) optimum temperature:
- 55° C. to 60° C.;
- and
- (4) thermal stability:
- stable at 55° C. or lower (pH 6.0, for 30 minutes).
A nucleosidase having the following enzymological properties:
- (1) action:
- catalyzing a reaction of hydrolyzing purine nucleosides into D-ribose and purine bases and showing activity even in the presence of adenosine, adenine, inosine, hypoxanthine, guanosine, guanine, and xanthine;
- (2) molecular weight:
- about 40 kDa (by SDS-PAGE) when the nucleosidase does not contain N-linked oligosaccharides;
- (3) optimum temperature:
- 50° C. to 55° C.;
- and
- (4) thermal stability:
- stable at 65° C. or lower (pH 4.5, for 60 minutes).
A nucleosidase gene comprising any DNA selected from the group consisting of the following (a) to (c):
- (a) a DNA encoding an amino acid sequence of SEQ ID NO:
- 1 or 2;
- (b) a DNA consisting of a base sequence of any of SEQ ID NOs:
- 3 to 6;
- and
- (c) a DNA having a base sequence equivalent to the base sequence of any of SEQ ID NOs:
- 3 to 6 and encoding a protein having nucleosidase activity.
Dependencies are inferred from the claim text.