An enzyme exhibiting fructan hydrolase activity
AssigneeOy Karl Fazer Ab
InventorsJussi Loponen, Markku Mikola, Juhani Sibakov
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Abstract
The present invention is related to an enzyme that allows efficient removal of fructan from grain and vegetable raw material. The enzyme according to the invention produces grain and vegetable material having a fructan content significantly lower compared to that of the starting material.
Claims23
A DNA construct comprising a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1.
A recombinant expression vector comprising a DNA construct, wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No 1.
A cell comprising a recombinant expression vector, wherein the recombinant expression vector comprises a DNA construct and .wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1.
A method of producing an enzyme exhibiting fructan hydrolase activity, the method comprising culturing a cell a under conditions permitting the production of the enzyme, and recovering the enzyme from the culture, wherein the cell comprises a recombinant expression vector, wherein the recombinant expression vector comprises a DNA construct, and wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown In SEQ ID No. 1.
An enzyme exhibiting fructan hydrolase activity, wherein the enzyme is encoded by a DNA construct or produced by a method of producing an enzyme exhibiting fructan hydrolase activity,
- wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1, and
- wherein the method of producing an enzyme exhibiting fructan hydrolase activity comprises culturing a ceil tinder conditions permitting the production of the enzyme, and recovering the enzyme from the culture, wherein the cell comprises a recombinant expression vector, wherein the recombinant expression vector comprises a DNA construct. and wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1.
An enzyme exhibiting fructan hydrolase activity, which enzyme comprises a polypeptide having an amino acid sequence essentially as shown in SEQ ID. No. 2.
An enzyme preparation for the degradation of fructan, said preparation comprising an enzyme exhibiting fructan hydrolase activity, together with carriers and/or emulsifiers,
- wherein the enzyme is encoded by a DNA construct or produced by a method of producing an enzyme exhibiting fructan hydrolase activity,
- wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID 1, and
- wherein the method of producing an enzyme exhibiting fructan hydrolase activity comprises culturing a cell under conditions permitting the production of the enzyme, and recovering the enzyme from the culture, wherein the ceil comprises a recombinant expression vector, wherein the recombinant expression vector comprises a DNA construct, and wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1.
Use of an enzyme exhibiting fructan hydrolase activity or an enzyme preparation for the degradation of fructan in grain materials or in vegetables,
- wherein the enzyme is encoded by a DNA construct, or produced by a method of producing an enzyme exhibiting fructan hydrolase activity.
- wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1, and
- wherein the method of producing an enzyme exhibiting fructan hydrolase activity comprises culturing a cell under conditions permitting the production of the enzyme. and recovering the enzyme from the culture, wherein the ceil comprises a recombinant expression vector, wherein the recombinant expression vector comprises a DNA construct and wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No 1.
- wherein the enzyme preparation comprises an enzyme exhibiting fructan hydrolase activity, together with carriers and/or emulsifiers,
- wherein the enzyme is encoded by a DNA construct or produced by a method of producing an enzyme exhibiting fructan hydrolase activity,
- wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase. wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No 1, and
- wherein the method of producing an enzyme exhibiting fructan hydrolase activity comprises culturing a cell under conditions permitting the production of the enzyme, and recovering the enzyme from the culture wherein the cell comprises a recombinant expression vector, wherein the recombinant expression vector comprises a DNA construct and wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1.
Use of an enzyme or an enzyme preparation for the preparation of baked products.
- wherein the enzyme is encoded by a DNA construct or produced by a method of producing an enzyme exhibiting fructan hydrolase activity,
- wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1, and
- wherein the method of producing an enzyme exhibiting fructan hydrolase activity comprises culturing a cell under conditions pertaining the production of the enzyme, and recovering the enzyme from the culture, wherein the cell comprises a recombinant expression vector, wherein the recombinant expression vector comprises a DNA construct and wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1,
- wherein the enzyme preparation comprises an enzyme exhibiting fructan hydrolase activity, together with carriers and/or emulsifiers,
- wherein the enzyme is encoded by a DNA construct or produced by a method of producing an enzyme exhibiting fructan hydrolase activity,
- wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1, and
- wherein the method of producing an enzyme exhibiting fructan hydrolase activity comprises culturing a cell under conditions permitting the production of the enzyme, and recovering the enzyme from the culture, wherein the cell comprises a recombinant expression vector, wherein the recombinant expression vector comprises a DNA construct, and wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1.
(canceled)
A premix or improver for baking, comprising an enzyme or an enzyme preparation
- wherein the enzyme is encoded by a DNA construct or produced by a method of producing an enzyme exhibiting fructan hydrolase activity,
- wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1, and
- wherein the method of producing an enzyme exhibiting fructan hydrolase activity comprises culturing a cell under conditions permitting the production of the enzyme, and recovering the enzyme from the culture, wherein the cell comprises a recombinant expression vector, wherein the recombinant expression vector comprises a DNA construct and wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1,
- wherein the enzyme preparation comprises an enzyme exhibiting fructan hydrolase activity, together with earners and/or emulsifiers,
- wherein, the enzyme is encoded by DNA construct or produced by a method of producing an enzyme exhibiting fructan hydrolase activity,
- wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase. wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1, and
- wherein the method of producing an enzyme exhibiting fructan hydrolase activity comprises culturing a cell under conditions permitting the production of the enzyme, and recovering the enzyme from the culture, wherein the cell comprises a recombinant expression vector., wherein the recombinant expression vector comprises a DNA construct, and wherein the DNA construct comprises a nucleotide sequence encoding an extracellular fructosidase, wherein said nucleotide sequence comprises the nucleotide sequence shown in SEQ ID No. 1 or an analogous sequence thereof having at least 96% identity to the nucleotide sequence shown in SEQ ID No. 1.
(canceled)
Dependencies are inferred from the claim text.