# US 2019/0170764 A1 · Methods and compositions for mass spectrometry analysis

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- Number: US 2019/0170764 A1
- Document ID: US20190170764A1
- Status: Published application
- Kind: A1
- Application number: 16/253,142
- Filed: 2019-01-21
- Published: 2019-06-06
- Earliest claimed: 2003-01-13
- Inventors: Tianxin Wang
- Web page: https://patentgrep.com/patent/US20190170764A1

## CPC

- G01N 33/6851: {Methods of protein analysis involving laser desorption ionisation mass spectrometry}
- H01J 49/164: {Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI] (sample holders H01J49/0418)}
- G01N 2560/00: Chemical aspects of mass spectrometric analysis of biological material
- Y10T 436/24: Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

Methods and compounds are provided to improve the desorption and ionization of analyte for mass spectrometry analysis. More specifically, it is for electrospray ionization (ESI) mass spectrometry. The method uses charged affinity molecules that can bind with analyte either temporarily or permanently to improve the desorption and ionization of analyte. The charged affinity molecules can be positively charged or negatively charged.

## Claims

### Claim 1

A method to improve the detection of non-ionizable analyte molecule in electrospray ionization (ESI) mass spectrometry, comprising:
providing permanently charged affinity molecule having affinity moiety selected from cyclodextrin , calixarene, cucurbituril and pillararene that can bind with said analyte molecule via non-covalent bond;
mixing said charged affinity molecule with a sample solution containing said analyte to form a solution containing a non-covalently bound complex between said analyte molecule and said charged affinity molecule; and
performing electrospray ionization (ESI) mass spectrometry for the solution containing the bound complex , and
detecting the presence of the analyte molecule by detecting the presence of the bound complex in ESI mass spectrometry.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the charged affinity molecule is positively charged.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the charged affinity molecule is negatively charged.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the charged affinity molecule contains quaternary ammonium cation group.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the charged affinity molecule contains sulfate anion group.

### Claim 6

A compound to improve the signal of non-ionizable analyte molecule in electrospray ionization (ESI) mass spectrometry comprising a permanently charged motif and a binding motif selected from cyclodextrin, calixarene, cucurbituril and pillararene that can bind with the said analyte molecule to form a non-covalent complex.

### Claim 7 (depends on 6)

The compound according to claim 6, wherein the charged motif is a positively charged group.

### Claim 8 (depends on 6)

The compound according to claim 6, wherein the charged motif is negatively charged group.

### Claim 9 (depends on 6)

The compound according to claim 6, wherein the charged motif is a quaternary ammonium cation group.

### Claim 10 (depends on 6)

The compound according to claim 6, wherein the charged motif is sulfate anion group.