# US 2019/0170743 A1 · Bimetal-conductive polymer janus composite nanostructure having electrical stimulation responsiveness, colloidal self-assembled structure thereof, preparation method thereof and biosensing, bioimaging, drug delivery and industrial application using the same

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2019/0170743 A1
- Document ID: US20190170743A1
- Status: Published application
- Kind: A1
- Application number: 16/324,278
- Filed: 2017-08-09
- Published: 2019-06-06
- Earliest claimed: 2016-08-09
- Assignees: Industry-University Cooperation Foundation Hanyang University Erica Campus
- Applicants: Industry-University Cooperation Foundation Hanyang University Erica Campus
- Inventors: Dong Woo LIM; Eun Young HWANG
- Web page: https://patentgrep.com/patent/US20190170743A1

## CPC

- G01N 33/54346: {Nanoparticles}
- A61K 9/5115: {Inorganic compounds}
- G01N 33/533: with fluorescent label
- G01N 33/54373: {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings}
- A61K 9/5146: {obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, polyamines, polyanhydrides}
- G01N 21/658: {enhancement Raman, e.g. surface plasmons}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The present disclosure relates to a bimetal-conductive polymer Janus composite nanostructure having electrical stimulation responsiveness, a colloidal self-assembled structure thereof, a preparation method thereof and biosensing, bioimaging, drug delivery and industrial application using the same.

## Claims

### Claim 1

A self-assembled bimetal-polymer Janus nanostructure comprising:
a bimetal nanocluster core; and
a conductive polymer shell located radially around the core.

### Claim 2 (depends on 1)

The self-assembled bimetal-polymer Janus nanostructure according to claim 1, wherein the bimetal nanocluster core comprises a first metal and a second metal surrounding the surface of the first metal, wherein the first metal and the second metal are respectively selected from a group consisting of silver, gold, copper and a mixture thereof and the first metal and the second metal are not identical.

### Claim 3 (depends on 1)

The self-assembled bimetal-polymer Janus nanostructure according to claim 1, wherein the conductive polymer is at least one selected from a group consisting of polypyrrole, polythiophene, poly(3,4-ethylenedioxythiophene) (PEDOT) and polyaniline.

### Claim 4 (depends on 1)

The self-assembled bimetal-polymer Janus nanostructure according to claim 1, wherein the bimetal nanocluster core further comprises a Raman dye.

### Claim 5 (depends on 4)

A metal nanoprobe for biosensing and/or bioimaging measurement based on surface-enhanced Raman scattering (SERS) using the Janus nanostructure according to claim 4.

### Claim 6 (depends on 1)

A fluorescence-based metal nanoprobe for biosensing and/or bioimaging measurement using the Janus nanostructure according to any of claim 1.

### Claim 7 (depends on 1)

A drug delivery system using the Janus nanostructure according to any of claim 1.

### Claim 8

A method for preparing a self-assembled bimetal-polymer Janus nanostructure, comprising:
i) preparing a metal nanoparticle forming a seed;
ii) adding the seed metal nanoparticle to an aqueous solution in which a conductive polymer monomer and a surfactant are dissolved;
iii) conducting an oxidation-reduction reaction between a metal ion and the conductive polymer monomer by adding a metal ion solution to the solution in which the seed metal nanoparticle is added in ii);
iv) preparing a bimetal-polymer Janus nanoparticle by forming a bimetal nanoparticle part as the metal ion is reduced by receiving an electron donated by the conductive polymer and is deposited on the surface of the seed metal nanoparticle and forming a conductive polymer part asymmetrically as the conductive polymer monomer is oxidized, is deposited on only one side of the bimetal nanoparticle part and grows into a conductive polymer;
v) adding ODA (octadecylamine) to a solution comprising the Janus nanoparticle; and
vi) forming a bimetal nanocluster core and a polymer shell located radially around the core through self-assembly as the bimetal nanoparticle in the Janus nanoparticle is covalently bonded to the ODA.

### Claim 9 (depends on 8)

The method for preparing a self-assembled bimetal-polymer Janus nanostructure according to claim 8, which further comprises, after iv), attaching a Raman dye on the surface of the bimetal nanoparticle.

### Claim 10 (depends on 8)

The method for preparing a self-assembled bimetal-polymer Janus nanostructure according to claim 8, wherein the seed metal in i) is selected from a group consisting of gold, silver, coper and a mixture thereof and the metal ion in iii) is selected from a group consisting of gold ion, silver ion, copper ion and a mixture thereof.

### Claim 11 (depends on 8)

The method for preparing a self-assembled bimetal-polymer Janus nanostructure according to claim 8, wherein the conductive polymer in ii) is at least one selected from a group consisting of polypyrrole, polythiophene, poly(3,4-ethylenedioxythiophene) (PEDOT) and polyaniline.

### Claim 12 (depends on 8)

The method for preparing a self-assembled bimetal-polymer Janus nanostructure according to claim 8, wherein the growth into the conductive polymer in iv) is achieved by surface-templated polymerization.

### Claim 13 (depends on 5)

A method for detecting a target material based on surface-enhanced Raman scattering (SERS), comprising:
a) preparing a sample solution comprising a target material to be detected;
b) immobilizing a first antibody for the target material onto a magnetic nanoparticle;
c) immobilizing a second antibody for the target material onto the metal nanoprobe according to claim 5;
d) forming an immune complex wherein the target material and the first antibody of the magnetic nanoparticle are conjugated by adding the first antibody-immobilized magnetic nanoparticle of b) to the sample solution of a);
e) forming a sandwich immune complex of the second antibody of the metal nanoprobe, the target material and the first antibody of the magnetic nanoparticle by adding the second antibody-immobilized metal nanoprobe of c) to the solution comprising the first antibody-conjugated immune complex of d);
f) separating the magnetic nanoparticle and the metal nanoprobe not forming the sandwich immune complex using a magnetic field; and
g) measuring a Raman signal of the sandwich immune complex.

### Claim 14 (depends on 13)

The method for detecting a target material based on surface-enhanced Raman scattering (SERS) according to claim 13, wherein the target material is a protein or a pathogen.

### Claim at position 15

15-68. (canceled)