# US 2019/0170712 A1 · Analysis device

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- Number: US 2019/0170712 A1
- Document ID: US20190170712A1
- Status: Published application
- Kind: A1
- Application number: 16/307,650
- Filed: 2016-06-28
- Published: 2019-06-06
- Earliest claimed: 2016-06-28
- Assignees: Shimadzu Corporation
- Applicants: Shimadzu Corporation
- Inventors: Manabu UEDA; Jun WATANABE
- Web page: https://patentgrep.com/patent/US20190170712A1

## CPC

- G01N 30/8631: {Peaks}
- G01N 30/72: Mass spectrometers {(mass spectrometers per se H01J49/00)}
- G01N 30/86: Signal analysis
- G01N 30/8641: {Baseline}
- G01N 30/8679: {Target compound analysis, i.e. whereby a limited number of peaks is analysed}
- G01N 30/7206: {interfaced to gas chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04)}
- G01N 30/7233: {interfaced to liquid or supercritical fluid chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04)}
- G01N 2030/625: {by measuring reference material, e.g. carrier without sample}
- G01N 2030/8872: {impurities}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The detection of minute amounts of components that have been undetectable due to an influence of a mobile phase or reagents or the like added to the mobile phase is realized in LC-MS. At the outset, blank measurement is executed, and an m/z value MBG of a background signal derived from a mobile phase or the like is extracted on a mass spectrum obtained by the blank measurement (S2-S4). An analysis method is then created that executes scanning measurement in a plurality of divided m/z ranges in which the m/z value MBG of the background signal has been excluded from a predetermined m/z range. An LC/MS analysis of the target sample is executed according to the analysis method (S5-S6). When a total ion chromatogram (TIC) is created from data obtained by the LC/MS analysis, influence of the background signal hardly appears in the TIC, and the base line is lowered.

## Claims

### Claim at position 1

1-10. (canceled)

### Claim 11

An analysis device comprising a mass spectrometer capable of obtaining a spectrum in a predetermined mass to charge ratio value range by executing an analysis according to an analysis method including analysis conditions, the analysis device being configured to detect, with the mass spectrometer, a component temporally separated by a chromatograph, the analysis device comprising:
a) a background information storage unit that stores a mass to charge ratio value of a background signal that appears in analysis; and
b) an analysis method creation unit that creates, wherein one or a plurality of mass to charge ratio values or a mass to charge ratio value range are set as one of the analysis conditions, an analysis method in which one or a plurality of mass to charge ratio values or a mass to charge ratio value range obtained by removing the mass to charge ratio value stored in the background information storage unit from the set mass to charge ratio value or the set mass to charge ratio value range are used as an analysis object;
c) a chromatogram acquisition unit that creates a total ion chromatogram based on results obtained by an analysis of a target sample according to the analysis method; and
d) a peak detector that detects a peak in which the proportion of a signal intensity on the total ion chromatogram to a signal intensity of a main peak on the chromatogram is equal to or larger than a predetermined threshold value.

### Claim 12 (depends on 11)

The analysis device according to claim 11, wherein
the peak detector is an impurity detector that detects a peak, in which the proportion of a signal intensity on the total ion chromatogram to a signal intensity of a main peak on the chromatogram is equal to or larger than a predetermined threshold value, as an impurity peak corresponding to an impurity contained in the target sample.

### Claim 13 (depends on 11)

The analysis device according to claim 11, further comprising
a background information acquiring unit that extracts a mass to charge ratio value of a background signal based on results obtained by a blank analysis and stores the extracted mass to charge ratio value in the background information storage unit.

### Claim 14 (depends on 11)

The analysis device according to claim 11, wherein
the background information storage unit is a database that stores mass to charge ratio values of background signals under various conditions.

### Claim 15 (depends on 11)

The analysis device according to claim 11, wherein
the analysis method creation unit creates, when one or a plurality of mass to charge ratios or a mass to charge ratio range are set as a repetitive analysis object as one of the analysis conditions, an analysis method in which one or a plurality of mass to charge ratios or a mass to charge ratio range obtained by removing the mass to charge ratio stored in the background information storage unit from the set mass to charge ratio or the set mass to charge ratio range are used as a repetitive analysis object.

### Claim 16

An analysis device including a mass spectrometer capable of obtaining a spectrum in a predetermined mass to charge ratio value range, the analysis device being configured to detect, with the mass spectrometer, a component temporally separated by a chromatograph, the analysis device comprising:
a) an exclusion information setting unit that sets a mass to charge ratio value or a mass to charge ratio value range to be excluded from an analysis object; and
b) an intensity value determination unit that removes the mass to charge ratio value or the mass to charge ratio value range set by the exclusion information setting unit, on a spectrum obtained by an analysis of a target sample, determines whether or not there is a peak having the signal intensity value that is larger than a predetermined threshold value with the mass to charge ratio value remaining in the mass to charge ratio value to determine a mass to charge ratio value at which a peak has the signal intensity value that is larger than the threshold value
c) a chromatogram creation unit that creates an extraction ion chromatogram at a mass to charge ratio value obtained in the intensity value determination unit using data obtained by an analysis of the target sample,
wherein the peak is detected with the extraction ion chromatogram.

### Claim 17 (depends on 16)

The analysis device according to claim 16, wherein
an impurity peak corresponding to an impurity contained in the target sample is detected on the extraction ion chromatogram.

### Claim 18 (depends on 16)

The analysis device according to claim 16, the analysis device being configured to execute an analysis according to an analysis method including analysis conditions, the analysis device further comprising
an analysis method creation unit that creates an analysis method in which a mass to charge ratio value obtained by the intensity value determination unit is used as a repetitive analysis object.

### Claim 19 (depends on 16)

The analysis device according to claim 16, wherein
the intensity value determination unit further includes an analysis control unit that determines a mass to charge ratio value at which a peak has the signal intensity value that is larger than the threshold value, based on a mass spectrum obtained in a substantially real time during execution of an analysis of a target sample, and that when the mass to charge ratio value has been obtained, controls each unit to execute SIM measurement or MRM measurement with the mass to charge ratio value being a target.