# US 2019/0170689 A1 · Mass spectrometer with tandem ion mobility analyzers

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- Number: US 2019/0170689 A1
- Document ID: US20190170689A1
- Status: Published application
- Kind: A1
- Application number: 16/268,214
- Filed: 2019-02-05
- Published: 2019-06-06
- Earliest claimed: 2017-05-24
- Applicants: Bruker Daltonik GmbH
- Inventors: Thomas BETZ; Melvin Andrew PARK; Mark RIDGEWAY
- Web page: https://patentgrep.com/patent/US20190170689A1

## CPC

- G01N 27/623: combined with mass spectrometry
- H01J 49/0031: {Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence)}
- H01J 49/004: {Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H)

## Abstract

The invention proposes a mass spectrometer comprising two ion mobility analyzers in tandem arrangement, of which at least one is a trapped ion mobility spectrometer (TIMS), and an ion gate which is located between the two ion mobility analyzers, and use thereof wherein ions are selectively transferred between the two ion mobility analyzers by adjusting the transmission of the ion gate while ions are separated in time according to ion mobility in the first ion mobility analyzer.

## Claims

### Claim 1

A method for analyzing ions in a mass spectrometer comprising an ion source, first and second trapped ion mobility spectrometry (TIMS) analyzers, a mass filter located between the first and second TIMS analyzers and a mass analyzer located downstream of the second TIMS analyzer, the method comprising the steps of:
transferring ions from the ion source to the first TIMS analyzer;
driving the ions transferred to the first TIMS analyzer by a first gas flow against a first counter-acting electric DC field barrier such that the ions are trapped and spatially separated according to their mobilities at different positions along a ramp of the first electric field barrier at which a friction force of the first gas flow equals the counter-acting force of the first electric DC field barrier;
temporally separating ions according to mobility in the first TIMS analyzer by adjusting a height of the first electric DC field barrier or the velocity of the first gas flow;
separating undesirable ion mobility species from ion mobility species of interest by varying the transmitted mass range of the mass filter while the ions are separated in the first TIMS analyzer;
transferring the filtered ions to the second TIMS analyzer;
driving the ions transferred to the second TIMS analyzer by a second gas flow against a second counter-acting electric DC field barrier such that the ions are trapped and spatially separated according to their mobilities at different positions along a ramp of the second electric DC field barrier at which a friction force of the second gas flow equals the counter-acting force of the second electric DC field barrier; and
separating the transferred ions according to mobility in the second TIMS analyzer by adjusting a height of the second electric DC field barrier or the velocity of the gas flow.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the mass filter is operated at a gas pressure above 10 Pascal.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the mass filter is operated at a gas pressure of one of or both TIMS analyzers.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the undesirable ion mobility species are singly charged.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the undesirable ion mobility species are chemical background.

### Claim 6 (depends on 1)

The method according to claim 1, wherein the ions which are separated in the second TIMS analyzer are further analyzed by acquiring mass spectra or fragment mass spectra.

### Claim 7

A mass spectrometer comprising an ion source, first and second trapped ion mobility spectrometry analyzers (TIMS), a mass filter located between the two ion mobility analyzers and a mass analyzer,
wherein the first and second TIMS analyzers each comprise a gas flow and a counter-acting electric DC field barrier configured to spatially separate ions according to their mobilities at different positions along a ramp of the electric field barrier at which a friction force of the first gas flow equals the counter-acting force of the electric DC field barrier and to temporally separate ions according to mobility by adjusting a height of the electric DC field barrier or a velocity of the gas flow, and
wherein the mass filter is configured to vary the transmitted mass range during operation of the first TIMS analyzer.

### Claim 8 (depends on 7)

The mass spectrometer according to claim 7, wherein the mass filter is one of a low-pass filter, a high-pass filter and a band-pass filter.

### Claim 9 (depends on 8)

The mass spectrometer according to claim 8, wherein the mass filter is operated at a gas pressure above 10 Pascal.

### Claim 10 (depends on 8)

The mass spectrometer according to claim 8, wherein the mass filter is a Loeb-Eiber mass filter.

### Claim 11 (depends on 7)

The mass spectrometer according to claim 7, further comprising a fragmentation cell between the second TIMS analyzer and the mass analyzer.