# US 2019/0170683 A1 · Thin layer chromatography plate and sample analysis method using same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0170683 A1 - Document ID: US20190170683A1 - Status: Published application - Kind: A1 - Application number: 16/323,532 - Filed: 2017-12-13 - Published: 2019-06-06 - Earliest claimed: 2016-12-26 - Assignees: Panasonic Intellecutal Property Management Co., Ltd. - Applicants: Panasonic Intellecutal Property Management Co., Ltd. - Inventors: TOSHIHIRO SAKAMOTO; CHIAKI SHIDO - Web page: https://patentgrep.com/patent/US20190170683A1 ## CPC - G01N 27/447: using electrophoresis - G01N 30/26: Conditioning of the fluid carrier; Flow patterns - G01N 30/88: Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 - G01N 30/90: Plate chromatography, e.g. thin layer or paper chromatography - G01N 30/92: Construction of the plate - G01N 30/94: Development - G01N 33/543: with an insoluble carrier for immobilising immunochemicals - G01N 2030/885: {involving polymers} - G01N 2030/945: {Application of reagents to undeveloped plate} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Provided is a thin layer chromatography plate that makes it possible to separate a plurality of components from each other more simply and more quickly. Thin layer chromatography plate includes substrate and separation layer that is disposed on substrate for separating multiple components included in a sample from each other. Separation layer has first layer that has a band shape and extends in first development direction and second layer that extends in second development direction orthogonal to first development direction. Second layer is in contact with first layer. First layer includes a hydrophilic porous body. Second layer includes a hydrophobic porous body. ## Claims ### Claim 1 A thin layer chromatography plate comprising: a substrate; and a separation layer disposed on the substrate, the separation layer separating multiple components included in a sample from each other, wherein the separation layer has a first layer that has a band shape and extends in a first development direction and a second layer that extends in a second development direction orthogonal to the first development direction, the second layer is in contact with the first layer, the first layer includes a hydrophilic porous body, and the second layer includes a hydrophobic porous body. ### Claim 2 (depends on 1) The thin layer chromatography plate according to claim 1, wherein the first layer and the second layer are both disposed on the substrate, and a lateral surface of the first layer and a lateral surface of the second layer are in contact with each other. ### Claim 3 (depends on 1) The thin layer chromatography plate according to claim 1, wherein the separation layer further has a third layer in contact with the second layer, the first layer, the second layer, and the third layer are arrayed in sequence in the second development direction, the third layer includes a porous body, and at least one requirement selected from among a requirement of a composition of the third layer being different from a composition of the second layer and a requirement of a structure of the third layer being different from a structure of the second layer is satisfied. ### Claim 4 (depends on 3) The thin layer chromatography plate according to claim 3, wherein the first layer, the second layer, and the third layer are disposed on the substrate, and the lateral surface of the first layer and the lateral surface of the second layer are in contact with each other. ### Claim 5 (depends on 2) The thin layer chromatography plate according to claim 2, wherein the separation layer further includes a functional layer that extends in the second development direction and includes the hydrophobic porous body or another hydrophobic porous body, the functional layer is in contact with the first layer, and the functional layer, the first layer, and the second layer are arrayed in sequence in the second development direction. ### Claim 6 (depends on 1) The thin layer chromatography plate according to claim 1, wherein the second layer is disposed on the substrate, the first layer is disposed on the second layer, and a lower surface of the first layer and an upper surface of the second layer are in contact with each other. ### Claim 7 (depends on 6) The thin layer chromatography plate according to claim 6, wherein the first layer is located between one end and the other end of the second layer in the second development direction. ### Claim 8 (depends on 1) The thin layer chromatography plate according to claim 1, wherein the hydrophobic porous body is an aggregate of silica gel particles modified with a hydrophobic functional group. ### Claim 9 (depends on 1) The thin layer chromatography plate according to claim 1, further comprising a pair of electrodes disposed at both ends of the first layer in the first development direction. ### Claim 10 (depends on 1) A sample analysis method comprising: placing a sample onto the first layer of the thin layer chromatography plate according to claim 1; bringing an end of the first layer in the first development direction into contact with a first developing solvent; and changing an orientation of the thin layer chromatography plate to bring the thin layer chromatography plate into contact with a second developing solvent containing an organic solvent with the first layer being impregnated with the first developing solvent. ### Claim 11 (depends on 10) The sample analysis method according to claim 10, wherein the first developing solvent is an aqueous solution. ### Claim 12 (depends on 10) The sample analysis method according to claim 10, wherein the second developing solvent is a mixed solvent containing the organic solvent and water. ### Claim 13 (depends on 9) A sample analysis method comprising: placing a sample onto the first layer of the thin layer chromatography plate according to claim 9; applying a voltage to the pair of electrodes; and bringing the thin layer chromatography plate into contact with a developing solvent containing an organic solvent.